Influence of Switchgrass TDIF-like Genes on Arabidopsis Vascular Development.
Influence of Switchgrass TDIF-like Genes on Arabidopsis Vascular Development.
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柳枝稷 TDIF 样基因对拟南芥维管发育的影响。
DOI：
10.3389/fpls.2021.737219
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发表时间：
2021
影响因子：
5.6
通讯作者：
Zheng B
中科院分区：
文献类型：
--
作者：
Tian D;Tang J;Luo L;Zhang Z;Du K;Larkin RM;Shi X;Zheng B
作为CLAVATA 3（CLV 3）/胚周区（CLE）家族的成员，十二肽的气管元件分化抑制因子（TDIF）对植物的维管发育具有重要影响。然而，TDIF肽基序的多态性对活性的影响仍然知之甚少。模式植物拟南芥为研究TDIF同源物的活性提供了一种快速有效的工具。研究了柳枝稷（Panicum virgatum）中93个CLE基因的5个TDIF同源基因，命名为PvTDIF-like（PvTDIFL）基因。PvTDIFL 1、PvTDIFL 3 MR 3和PvTDIFL 3 MR 2的表达水平相对较高，并且它们都在柳枝稷的轴中以最高水平表达。PvTDIFL 1、PvTDIFL 3 MR 3和PvTDIFL 3 MR 2的前体蛋白分别含有一个、三个和两个TDIFL基序。用外源PvTDIFL肽处理增加了拟南芥幼苗下胚轴中柱细胞的数量，但PvTDIFL_4p除外。拟南芥中PvTDIFL 1的异源表达强烈抑制植物生长，增加下胚轴维管组织中的细胞分裂，并破坏下胚轴的细胞组织。尽管PvTDIFL 3 MR 3和PvTDIFL 3 MR 2的异源表达也影响植物生长和维管发育，但PvTDIFL 3 MR 3和PvTDIFL 3 MR 2编码的多个TDIFL基序并不增强PvTDIFL活性。这些数据表明，在一般情况下，PvTDIFL是功能上类似于拟南芥TDIF，但PvTDIFL肽的加工和活动更复杂。
As a member of the CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (CLE) family, the dodecapeptide tracheary element differentiation inhibitory factor (TDIF) has a major impact on vascular development in plants. However, the influence of polymorphisms in the TDIF peptide motif on activity remains poorly understood. The model plant, Arabidopsis provides a fast and effective tool for assaying the activity of TDIF homologs. Five TDIF homologs from a group of 93 CLE genes in switchgrass (Panicum virgatum), a perennial biomass crop, named PvTDIF-like (PvTDIFL) genes were studied. The expression levels of PvTDIFL1, PvTDIFL3MR3, and PvTDIFL3MR2 were relatively high and all of them were expressed at the highest levels in the rachis of switchgrass. The precursor proteins for PvTDIFL1, PvTDIFL3MR3, and PvTDIFL3MR2 contained one, three, and two TDIFL motifs, respectively. Treatments with exogenous PvTDIFL peptides increased the number of stele cells in the hypocotyls of Arabidopsis seedlings, with the exception of PvTDIFL_4p. Heterologous expression of PvTDIFL1 in Arabidopsis strongly inhibited plant growth, increased cell division in the vascular tissue of the hypocotyl, and disrupted the cellular organization of the hypocotyl. Although heterologous expression of PvTDIFL3MR3 and PvTDIFL3MR2 also affected plant growth and vascular development, PvTDIFL activity was not enhanced by the multiple TDIFL motifs encoded by PvTDIFL3MR3 and PvTDIFL3MR2. These data indicate that in general, PvTDIFLs are functionally similar to Arabidopsis TDIF but that the processing and activities of the PvTDIFL peptides are more complex.
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影响因子：
11.6
作者：
Fiers, M;Golemiec, E;Liu, CM
通讯作者：
Liu, CM
影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
影响因子：
56.9
作者：
Kondo, Tatsuhiko;Sawa, Shinichiro;Sakagami, Youji
通讯作者：
Sakagami, Youji
DOI：
10.1007/978-1-4939-8778-8_8
发表时间：
2019-01-01
期刊：
TRANSGENIC PLANTS
影响因子：
--
作者：
Chen, QiuXia;Song, Guo-Qing
通讯作者：
Song, Guo-Qing