Species diversity and relative abundance of lactic acid bacteria in the milk of rhesus monkeys (Macaca mulatta).
Species diversity and relative abundance of lactic acid bacteria in the milk of rhesus monkeys (Macaca mulatta).
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DOI：
10.1111/j.1600-0684.2010.00450.x
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发表时间：
2011-02
影响因子：
0.7
通讯作者：
Tao L
中科院分区：
文献类型：
--
作者：
Jin L;Hinde K;Tao L
母乳是影响婴儿共生肠道菌群发育的细菌来源。迄今为止，非人灵长类动物乳酸菌的种类多样性和相对丰度尚未得到描述。对54只哺乳期雌恒河猴（Macaca mulatta）的乳汁进行了无菌采集。GM17和MRS琼脂电泳后，根据形态差异分离单个菌落，然后根据SDS-PAGE上的全细胞蛋白谱进行分组。分离细菌DNA，分析16S rRNA基因序列。共鉴定出芽孢杆菌（Bacillus）、肠球菌（Enterococcus）、乳杆菌（Lactobacillus）、Pediococcus和链球菌（Streptococcus） 5属19种106株。母体肠道和口腔共生菌可能在哺乳期转移到乳腺并存在于乳汁中。这一途径可能是婴儿肠道和口腔共生细菌的重要来源。
Mother’s milk is a source of bacteria that influences the development of the infant commensal gut microbiota. To date, the species diversity and relative abundance of lactic acid bacteria in the milk of non-human primates have not been described. Milk samples were aseptically obtained from 54 female rhesus monkeys (Macaca mulatta) at peak lactation. Following GM17 and MRS agar plating, single bacterial colonies were isolated based on difference in morphotypes, then grouped based on whole-cell protein profiles on SDS–PAGE. Bacterial DNA was isolated and the sequence the 16S rRNA gene was analyzed. A total of 106 strains of 19 distinct bacterial species, belonging to five genera, Bacillus, Enterococcus, Lactobacillus, Pediococcus, and Streptococcus, were identified. Maternal gut and oral commensal bacteria may be translocated to the mammary gland during lactation and present in milk. This pathway can be an important source of commensal bacteria to the infant gut and oral cavity.
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影响因子：
4.4
作者：
Jimenez, E.;Fernandez, L.;Rodriguez, J. M.
通讯作者：
Rodriguez, J. M.
DOI：
10.1016/j.ijfoodmicro.2006.06.011
发表时间：
2006-10-15
影响因子：
5.4
作者：
Martin, R.;Jimenez, E.;Rodriguez, J. M.
通讯作者：
Rodriguez, J. M.
影响因子：
--
作者：
Morita, E;Narikiyo, M;Sasaki, H
通讯作者：
Sasaki, H
影响因子：
2.4
作者：
Hinde, Katie;Captanio, John P.
通讯作者：
Captanio, John P.
DOI：
10.1053/j.spid.2004.07.002
发表时间：
2004-10-01
期刊：
Seminars in pediatric infectious diseases
影响因子：
--
作者：
Morrow, Ardythe L;Rangel, Josefa M
通讯作者：
Rangel, Josefa M