High Prevalence and Factors Associated With the Distribution of the Integron intI1 and intI2 Genes in Scottish Cattle Herds.
High Prevalence and Factors Associated With the Distribution of the Integron intI1 and intI2 Genes in Scottish Cattle Herds.
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DOI：
10.3389/fvets.2021.755833
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发表时间：
2021
影响因子：
3.2
通讯作者：
Hoyle DV
中科院分区：
文献类型：
--
作者：
Fernández Rivas C;Porphyre T;Chase-Topping ME;Knapp CW;Williamson H;Barraud O;Tongue SC;Silva N;Currie C;Elsby DT;Hoyle DV
整合子是在阵列中捕获和表达抗菌素耐药性基因的遗传元件，促进多种细菌耐药性在一系列细菌物种中的水平传播。本研究的目的是估计苏格兰牛中 1、2 和 3 类整合子的患病率，并检查空间、季节或牛群管理因素是否影响整合子牛群状态。我们使用了 2014 年至 2015 年全国横断面调查中从 108 个苏格兰牛群收集的粪便样本，并通过多重 PCR 筛选粪便 DNA 提取物中的整合酶基因 intI1、intI2 和 intI3。群体水平患病率估计[95%置信区间 (CI)]，intI1 为 76.9% (67.8–84.0%)，intI2 为 82.4% (73.9–88.6%)。我们没有在任何测试的牛群样本中检测到 intI3。 intI1 观察到区域效应，东北部（OR 11.5，95% CI：1.0–130.9，P = 0.05）和东南部（OR 8.7，95% CI：1.1–20.9，P = 0.04）最高，高地最低。使用广义线性混合模型来测试畜群状况与牛管理、土壤类型和区域牲畜密度变量之间的潜在关联。在最终的多变量模型中，与 intI1 牛群阳性相关的因素包括一年中的春季（OR 6.3，95% CI：1.1–36.4，P = 0.04）和从天然泉水源给牛浇水（OR 4.4，95% CI：1.3–14.8，P = 0.017），以及在 intI2 采样时饲养的牛（OR 75.0，95% CI：10.4–540.5，P < 0.001）。这项研究提供了苏格兰牛中整合子流行率的基线估计，并确定了可能与携带相关的因素，值得未来研究。
Integrons are genetic elements that capture and express antimicrobial resistance genes within arrays, facilitating horizontal spread of multiple drug resistance in a range of bacterial species. The aim of this study was to estimate prevalence for class 1, 2, and 3 integrons in Scottish cattle and examine whether spatial, seasonal or herd management factors influenced integron herd status. We used fecal samples collected from 108 Scottish cattle herds in a national, cross-sectional survey between 2014 and 2015, and screened fecal DNA extracts by multiplex PCR for the integrase genes intI1, intI2, and intI3. Herd-level prevalence was estimated [95% confidence interval (CI)] for intI1 as 76.9% (67.8–84.0%) and intI2 as 82.4% (73.9–88.6%). We did not detect intI3 in any of the herd samples tested. A regional effect was observed for intI1, highest in the North East (OR 11.5, 95% CI: 1.0–130.9, P = 0.05) and South East (OR 8.7, 95% CI: 1.1–20.9, P = 0.04), lowest in the Highlands. A generalized linear mixed model was used to test for potential associations between herd status and cattle management, soil type and regional livestock density variables. Within the final multivariable model, factors associated with herd positivity for intI1 included spring season of the year (OR 6.3, 95% CI: 1.1–36.4, P = 0.04) and watering cattle from a natural spring source (OR 4.4, 95% CI: 1.3–14.8, P = 0.017), and cattle being housed at the time of sampling for intI2 (OR 75.0, 95% CI: 10.4–540.5, P < 0.001). This study provides baseline estimates for integron prevalence in Scottish cattle and identifies factors that may be associated with carriage that warrant future investigation.
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影响因子：
5.2
作者：
Chainier D;Barraud O;Masson G;Couve-Deacon E;François B;Couquet CY;Ploy MC
通讯作者：
Ploy MC
影响因子：
8.9
作者：
Di Cesare, Andrea;Eckert, Ester M.;Corno, Gianluca
通讯作者：
Corno, Gianluca
影响因子：
8.9
作者：
Dungan, Robert S.;Bjorneberg, David L.
通讯作者：
Bjorneberg, David L.
影响因子：
4.9
作者：
Gaze, WH;Abdouslam, N;Wellington, EMH
通讯作者：
Wellington, EMH
影响因子：
5.2
作者：
Barraud, O.;Baclet, M. C.;Ploy, M. C.
通讯作者：
Ploy, M. C.