Investigation into the stability and culturability of Chinese enterotypes.
Investigation into the stability and culturability of Chinese enterotypes.
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中国肠型稳定性及可培养性研究
DOI：
10.1038/s41598-017-08478-w
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发表时间：
2017-08-11
影响因子：
4.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Yin Y;Fan B;Liu W;Ren R;Chen H;Bai S;Zhu L;Sun G;Yang Y;Wang X
尽管在欧洲、非洲和美国已经报道了许多肠道微生物肠型，它们对人类健康的影响尚不清楚。肠道菌群的体外培养将有助于研究其作用和应用。在这里，收集了来自13名健康中国志愿者的粪便样本，并进行了下一代测序。结果表明，其中7份为类杆菌肠型，6份为普雷沃菌肠型。还评价了这些中国肠道微生物肠型的稳定性。结果显示，大多数受试志愿者的肠道菌群非常稳定。对于一名志愿者来说，细菌群落在四个月后恢复到肠道灌洗和抗生素治疗前的状态。发现XP培养基可有效模拟体外恒化器培养系统中不依赖于原始肠道微生物群落的拟杆菌肠型。虽然Prevotella肠型在体外模拟得不是很好，但不同的培养成分选择性地富集了不同的肠道细菌。果胶和木聚糖与类杆菌属、Sutterella和Flavonifractor在恒化培养系统中的富集有关。
Although many gut microbial enterotypes have been reported in Europe, Africa and the U.S., their effects on human health are still not yet clear. Culturing gut microbial enterotypesin vitrowill be helpful to study their effects and applications. Here, fecal samples from 13 healthy Chinese volunteers were collected and subjected to next-generation sequencing. The results showed that seven of these samples belong to theBacteroidesenterotype and another six to thePrevotellaenterotype. Stability of these Chinese gut microbial enterotypes was also evaluated. Results showed that most of the tested volunteer gut microbiota to be very stable. For one volunteer, the bacterial community returned to the state it was in before intestinal lavage and antibiotics treatment after four months. XP medium was found effective for simulating theBacteroidesenterotype independent of the original gut microbial community in anin vitrochemostat culture system. Although, thePrevotellaenterotype was not very well simulatedin vitro, different culture elements selectively enriched different gut bacteria. Pectin and xylan were found to be related to the enrichment of the generaBacteroides,Sutterella, andFlavonifractorin this chemostat culture system.
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影响因子：
4.6
作者：
Nakayama J;Watanabe K;Jiang J;Matsuda K;Chao SH;Haryono P;La-Ongkham O;Sarwoko MA;Sujaya IN;Zhao L;Chen KT;Chen YP;Chiu HH;Hidaka T;Huang NX;Kiyohara C;Kurakawa T;Sakamoto N;Sonomoto K;Tashiro K;Tsuji H;Chen MJ;Leelavatcharamas V;Liao CC;Nitisinprasert S;Rahayu ES;Ren FZ;Tsai YC;Lee YK
通讯作者：
Lee YK
影响因子：
4.2
作者：
Marzorati M;Vanhoecke B;De Ryck T;Sadaghian Sadabad M;Pinheiro I;Possemiers S;Van den Abbeele P;Derycke L;Bracke M;Pieters J;Hennebel T;Harmsen HJ;Verstraete W;Van de Wiele T
通讯作者：
Van de Wiele T
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM