New approach for m-cell-specific molecules screening by comprehensive transcriptome analysis.
New approach for m-cell-specific molecules screening by comprehensive transcriptome analysis.
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DOI：
10.1093/dnares/dsp013
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Ohno H
中科院分区：
文献类型：
--
作者：
Nakato G;Fukuda S;Hase K;Goitsuka R;Cooper MD;Ohno H
在肠Peyer’s patches （PPs）的滤泡相关上皮（FAE）内的少量M细胞群是外源抗原进入的主要入口。哺乳动物M细胞的表征，包括用于细菌摄取的M细胞表面分子的鉴定，由于其相对罕见而受到阻碍。相比之下，M细胞几乎构成了鸟类法氏囊的所有FAE细胞。因此，我们比较了鸡和小鼠FAE的基因表达谱，以鉴定两种物种中M细胞共同表达的基因。综合转录组分析显示，28个基因在两种物种的FAE中普遍上调。原位杂交结果显示，膜联蛋白A10 (Anxa10) mRNA分散在FAE中，并与Ulex europaeus凝集素-1共定位结合M细胞。全挂免疫染色还发现细胞朊蛋白（PrPC）表达在M细胞顶质膜的管腔侧，并与鼠PP中仅识别M细胞的grycoprotein 2共定位。我们的研究结果通过综合转录组分析发现了新的M细胞特异性分子。小鼠和鸡M细胞中的这些保守分子可能在M细胞功能和/或分化中起重要作用。
A minor population of M cells within the follicle-associated epithelium (FAE) of intestinal Peyer's patches (PPs) serves as a major portal for entry of exogenous antigens. Characterization of the mammalian M cells, including identification of M-cell surface molecules used for bacterial uptake, has been hampered by their relative rarity. In contrast, M cells constitute virtually all of the FAE cells in the avian bursa of Fabricius. We therefore performed comparative gene expression profiling of chicken and murine FAE to identify commonly expressed genes by M cells in both species. The comprehensive transcriptome analysis revealed that 28 genes were commonly up-regulated in FAE from both species. In situ hybridization revealed that annexin A10 (Anxa10) mRNA was scattered in FAE, and co-localized with Ulex europaeus agglutinin-1 binding to M cells. Whole-mount immunostaining also revealed that cellular prion protein (PrPC) was expressed on the luminal side of the apical plasma membrane of M cells, and co-localized with grycoprotein 2 that recognizes only M cells in murine PP. Our findings identify new M-cell-specific molecules through using comprehensive transcriptome analysis. These conserved molecules in M cells of mice and chickens may play essential roles in M-cell function and/or differentiation.
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