Incorporation of Natural and Recombinant Collagen Proteins within Fmoc-Based Self-Assembling Peptide Hydrogels.
Incorporation of Natural and Recombinant Collagen Proteins within Fmoc-Based Self-Assembling Peptide Hydrogels.
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DOI：
10.3390/gels8050254
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发表时间：
2022-04-21
期刊：
影响因子：
4.6
通讯作者：
Bella, Jordi
中科院分区：
文献类型：
--
作者：
Vitale, Mattia;Ligorio, Cosimo;Smith, Ian P.;Richardson, Stephen M.;Hoyland, Judith A.;Bella, Jordi
水凝胶生物材料通过其纳米纤维超微结构和组成模拟天然细胞外基质，并在其聚合物网络内为细胞-基质和细胞-细胞相互作用提供适当的环境。水凝胶可以用不同的蛋白质、细胞因子或细胞粘附基序修饰以控制细胞行为和细胞分化。由于胶原蛋白在脊椎动物细胞外基质中的丰富性及其与细胞表面受体的相互作用，胶原蛋白是水凝胶修饰的理想且多功能的蛋白质。在这里，我们报告了一种快速，廉价和有效的协议，将天然，合成和重组胶原蛋白纳入Fmoc为基础的自组装肽水凝胶。通过扩散方案对水凝胶进行改性，其中不同分子大小的胶原蛋白分子随着时间的推移被成功地掺入并保留。表征研究表明，这些胶原与水凝胶纤维相互作用，而不影响复合水凝胶的整体机械性能。此外，掺入水凝胶中的胶原分子仍具有生物活性，并通过与α2β1整联蛋白相互作用为人纤维肉瘤细胞的粘附和扩散提供位点。我们的方案可用于将不同类型的胶原蛋白分子掺入基于肽的水凝胶中，而无需任何事先的化学修饰。这些改性的水凝胶可用于需要胶原基底物来分化和控制细胞行为的研究。我们的方案可以很容易地适应于将其他生物活性蛋白质和肽掺入基于肽的水凝胶中，以调节它们的特性及其与不同细胞类型的相互作用。
Hydrogel biomaterials mimic the natural extracellular matrix through their nanofibrous ultrastructure and composition and provide an appropriate environment for cell–matrix and cell–cell interactions within their polymeric network. Hydrogels can be modified with different proteins, cytokines, or cell-adhesion motifs to control cell behavior and cell differentiation. Collagens are desirable and versatile proteins for hydrogel modification due to their abundance in the vertebrate extracellular matrix and their interactions with cell-surface receptors. Here, we report a quick, inexpensive and effective protocol for incorporation of natural, synthetic and recombinant collagens into Fmoc-based self-assembling peptide hydrogels. The hydrogels are modified through a diffusion protocol in which collagen molecules of different molecular sizes are successfully incorporated and retained over time. Characterization studies show that these collagens interact with the hydrogel fibers without affecting the overall mechanical properties of the composite hydrogels. Furthermore, the collagen molecules incorporated into the hydrogels are still biologically active and provide sites for adhesion and spreading of human fibrosarcoma cells through interaction with the α2β1 integrin. Our protocol can be used to incorporate different types of collagen molecules into peptide-based hydrogels without any prior chemical modification. These modified hydrogels could be used in studies where collagen-based substrates are required to differentiate and control the cell behavior. Our protocol can be easily adapted to the incorporation of other bioactive proteins and peptides into peptide-based hydrogels to modulate their characteristics and their interaction with different cell types.
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