Wakame seaweed suppresses the proliferation of 7,12-dimethylbenz(a)-anthracene-induced mammary tumors in rats.
Wakame seaweed suppresses the proliferation of 7,12-dimethylbenz(a)-anthracene-induced mammary tumors in rats.
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DOI：
10.1111/j.1349-7006.1999.tb00836.x
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发表时间：
1999-09
期刊：
影响因子：
--
通讯作者：
Narita T
中科院分区：
文献类型：
--
作者：
Funahashi H;Imai T;Tanaka Y;Tsukamura K;Hayakawa Y;Kikumori T;Mase T;Itoh T;Nishikawa M;Hayashi H;Shibata A;Hibi Y;Takahashi M;Narita T
本实验观察了海藻对7，12-二甲基苯并(A)-菲(DMBA)诱发的大鼠乳腺肿瘤的抑制作用。给8周龄雌性SD大鼠注射DMBA，并将形成乳腺肿瘤的大鼠随机分为三组。对照组(I-A组)用商品大鼠饲料，配制成两种饲料混合物：商品大鼠饲料中分别添加1.0%(I-B组)和5.0%(I-C组)的WAKAME。每组分别饲喂8周，比较各组乳腺肿瘤大小的变化。实验结束时，切除乳腺肿瘤和甲状腺组织，比较它们的重量。测定血清总碘和甲状腺激素(T4)水平。采用免疫组织化学方法对肿瘤组织进行溴脱氧尿苷标记、转化生长因子-β和细胞凋亡检测。与I-A组相比，I-B组和I-C组对肿瘤生长有明显的抑制作用。I-B组和I-C组切除的乳腺肿瘤重量明显低于I-A组，血清总碘浓度显著高于I-A组。这些结果表明，碘从血清转运到乳腺组织，并通过转化生长因子-β的表达诱导细胞凋亡。综上所述，WAKAME抑制了DMBA诱导的乳腺肿瘤的增殖。
We examined the anti‐tumor proliferation effects of wakame seaweed on 7,12‐dimethylbenz(a)‐anthracene (DMBA)‐induced rat mammary tumor. DMBA was administered to 8‐week‐old female Sprague‐Dawley rats, and rats which developed mammary tumors were assigned randomly to three groups. Commercial rat feed was used in a control group (group I‐A), and two feed mixtures were prepared, which contained commercial rat feed blended with wakame at 1.0% (group I‐B) and 5.0% (group I‐C) by weight. The respective feeds were given to each group for 8 weeks, and changes in mammary tumor size were compared. At the end of the experiment, mammary tumors and thyroid glands were resected to compare their weights. Serum total iodine and thyroxin (T4) levels were measured. Immunohistochemical studies for bromodeoxyuridine (BrdU) labeling, transforming growth factor (TGF)‐β, and apoptosis were carried out in the resected tumor. Significant suppression of tumor growth was observed in groups I‐B and I‐C compared with I‐A. In groups I‐B and I‐C, the weights of resected mammary tumors were significantly lower and serum total iodine concentration was significantly higher than in I‐A. BrdU indices were significantly lower in groups I‐B and I‐C, compared with I‐A. TGF‐β and apoptotic index were inversely related to BrdU. These results suggest that iodine is transported from the serum into mammary tissues and induces apoptosis through the expression of TGF‐β. In conclusion, wakame suppressed the proliferation of DMBA‐induced mammary tumors.
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影响因子：
64.5
作者：
KNABBE, C;LIPPMAN, ME;DICKSON, RB
通讯作者：
DICKSON, RB
影响因子：
9.7
作者：
YAMAMOTO, I;MARUYAMA, H;MORIGUCHI, M
通讯作者：
MORIGUCHI, M
影响因子：
5.4
作者：
SMYTH, PPA
通讯作者：
SMYTH, PPA
影响因子：
8.8
作者：
SICHER, K;WATERHOUSE, JA
通讯作者：
WATERHOUSE, JA
影响因子：
64.8
作者：
HUGGINS, C;BRILLANTES, FP;GRAND, LC
通讯作者：
GRAND, LC