2-Methyl-pentanoyl-carnitine (2-MPC): a urine biomarker for patent Ascaris lumbricoides infection.
2-Methyl-pentanoyl-carnitine (2-MPC): a urine biomarker for patent Ascaris lumbricoides infection.
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DOI：
10.1038/s41598-020-72804-y
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发表时间：
2020-09-25
影响因子：
4.6
通讯作者：
Stuyver LJ
中科院分区：
文献类型：
--
作者：
Lagatie O;Verheyen A;Van Asten S;Odiere MR;Djuardi Y;Levecke B;Vlaminck J;Mekonnen Z;Dana D;T'Kindt R;Sandra K;van Outersterp R;Oomens J;Lin R;Dillen L;Vreeken R;Cuyckens F;Stuyver LJ
蛔虫等肠道蠕虫感染影响着世界所有热带和亚热带地区的数亿人。通过大规模的驱虫项目，世界卫生组织的目标是将中度至重度感染减少到1%以下。目前对这些控制方案的诊断和监测仅仅是基于粪便中虫卵的检测。在这里，我们描述了如何使用代谢组学分析来鉴定A. lumbriicoides特异性尿液生物标志物2-甲基戊酰肉碱（2-MPC）。该生物标志物在确定感染方面的准确率为85.7%，在确定中度至重度感染方面的准确率为90.5%。我们的研究结果还表明，尿液中的2-MPC水平与粪便中检测到的类蚓蚓DNA之间存在相关性。此外，在给予标准治疗（单次口服400mg阿苯达唑）后，尿中2-MPC水平迅速而强烈地下降。在猪蛔虫感染模型中，研究发现，尽管2-MPC水平远低于人类，但尿中2-MPC水平与蠕虫计数（p = 0.023）和每克卵数（p < 0.001）之间存在显著关联。本报告表明，尿2-MPC可被认为是一种可用于监测感染强度的类蚓单胞菌特异性生物标志物。
Infections with intestinal worms, such as Ascaris lumbricoides, affect hundreds of millions of people in all tropical and subtropical regions of the world. Through large-scale deworming programs, World Health Organization aims to reduce moderate-to-heavy intensity infections below 1%. Current diagnosis and monitoring of these control programs are solely based on the detection of worm eggs in stool. Here we describe how metabolome analysis was used to identify the A. lumbricoides-specific urine biomarker 2-methyl pentanoyl carnitine (2-MPC). This biomarker was found to be 85.7% accurate in determining infection and 90.5% accurate in determining a moderate-to-heavy infection. Our results also demonstrate that there is a correlation between 2-MPC levels in urine and A. lumbricoides DNA detected in stool. Furthermore, the levels of 2-MPC in urine were shown to rapidly and strongly decrease upon administration of a standard treatment (single oral dose of 400 mg albendazole). In an Ascaris suum infection model in pigs, it was found that, although 2-MPC levels were much lower compared to humans, there was a significant association between urinary 2-MPC levels and both worm counts (p = 0.023) and the number of eggs per gram (epg) counts (p < 0.001). This report demonstrates that urinary 2-MPC can be considered an A. lumbricoides-specific biomarker that can be used to monitor infection intensity.
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影响因子：
4.8
作者：
Lagatie O;Van Loy T;Tritsmans L;Stuyver LJ
通讯作者：
Stuyver LJ
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影响因子：
4.8
作者：
Lagatie O;Van Loy T;Tritsmans L;Stuyver LJ
通讯作者：
Stuyver LJ
影响因子：
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作者：
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通讯作者：
Nutman, Thomas B.
影响因子：
2.1
作者：
HALL, A;ROMANOVA, T
通讯作者：
ROMANOVA, T
影响因子：
3.8
作者：
Cools, Piet;Vlaminck, Johnny;Levecke, Bruno
通讯作者：
Levecke, Bruno