Spared nerve injury rats exhibit thermal hyperalgesia on an automated operant dynamic thermal escape task.
Spared nerve injury rats exhibit thermal hyperalgesia on an automated operant dynamic thermal escape task.
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DOI：
10.1186/1744-8069-1-18
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发表时间：
2005-05-26
期刊：
影响因子：
3.3
通讯作者：
Apkarian AV
中科院分区：
文献类型：
--
作者：
Baliki M;Calvo O;Chialvo DR;Apkarian AV
成熟的方法可用于测量清醒行为大鼠的热敏感性和机械敏感性。然而，它们需要实验者的操作，并倾向于强调反射性行为。本文介绍一种新的行为学测试方法，用于检测神经病理性损伤大鼠的热敏感性。我们比较了保留神经损伤和慢性压迫性损伤大鼠的热痛敏。该设备是一种全自动热敏感性评估工具，旨在强调对动态施加到动物站立表面的热疼痛和非疼痛刺激的综合学习反应。它记录了清醒的、不受约束的动物对动物所在地板的无害和有害加热的逃避行为。动物学会通过逃避到相反的非加热侧来最小化疼痛;记录逃避潜伏期。在该装置上，两组神经病大鼠的热刺激-反应曲线均显示> 6°C的温度漂移。相比之下，当这些动物在热板上测试时，刺激-反应偏移< 2°C。通过红外线加热缩足试验、足底试验检测热敏感性，结果表明，坐骨神经损伤大鼠热痛觉过敏的证据更少。从所使用的测试以及所研究的动物模型的角度讨论了热痛觉过敏的测试依赖性幅度的含义。有人认为，动态热操作任务揭示了神经病理性损伤相关的疼痛样行为的相关性，与整个有机体。
Well-established methods are available to measure thermal and mechanical sensitivity in awake behaving rats. However, they require experimenter manipulations and tend to emphasize reflexive behaviors. Here we introduce a new behavioral test, with which we examine thermal sensitivity of rats with neuropathic injury. We contrast thermal hyperalgesia between spared nerve injury and chronic constriction injury rats. This device is a fully automated thermal sensitivity assessment tool designed to emphasize integrated learned responses to thermal painful and non-painful stimuli that are applied dynamically to a surface on which the animal is standing. It documents escape behavior in awake, unrestrained animals to innocuous and noxious heating of the floor where the animal is located. Animals learn to minimize pain by escaping to the opposite non-heated side; escape latency is recorded. On this device, thermal stimulus-response curves showed > 6°C leftward shift in both groups of neuropathic rats. In contrast, when these animals were tested on hotplate the stimulus-response shift was < 2°C. Spared nerve injury rats showed even less evidence for thermal hyperalgesia when thermal sensitivity was tested by measuring paw withdrawal to infrared heating, plantar test. The implications of test dependent magnitude of thermal hyperalgesia are discussed from the viewpoint of the tests used, as well as the animal models studied. It is argued that the dynamic thermal operant task reveals the relevance of the neuropathic injury associated pain-like behavior in relation to the whole organism.
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影响因子：
64.8
作者：
Konig, M;Zimmer, AM;Zimmer, A
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作者：
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