Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida.
Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida.
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DOI：
10.1093/jxb/ern216
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发表时间：
2008
影响因子：
6.9
通讯作者：
Elomaa P
中科院分区：
文献类型：
--
作者：
Laitinen RA;Ainasoja M;Broholm SK;Teeri TH;Elomaa P
类黄酮途径的遗传修饰已被用于在观赏植物中产生新的颜色和颜色图案，以及改变粮食作物的营养和药物特性。有人建议，在调节基因的帮助下协调控制该途径的多个步骤将导致对代谢通量的更可预测的控制。花青苷的生物合成调控已在一种常见的观赏植物，非洲菊（菊科）。R2R3型MYB因子GMYB10具有高度的序列相似性，并且与先前表征的花青素色素沉着调节剂在遗传学上分组在一起。GMYB10的异位表达导致转基因非洲菊植物中花色素苷色素的强烈增强的积累以及改变的色素沉着模式。花色素苷分析表明，GMYB10特异性地诱导未分化愈伤组织和营养组织中花青素的生物合成。此外，在花组织中检测到增强的天竺葵素产生。使用非洲菊9K cDNA阵列的微阵列分析揭示了一组高度预测的推定的靶基因GMYB10，包括新的基因家族成员的早期和晚期的类黄酮途径的生物合成基因。然而，还鉴定了全新的候选靶点，例如丝氨酸羧肽酶样基因，以及两个新的MYB结构域因子GMYB 11和GMYB 12，其在苯丙素类生物合成中的确切功能尚不清楚。
Genetic modification of the flavonoid pathway has been used to produce novel colours and colour patterns in ornamental plants as well as to modify the nutritional and pharmaceutical properties of food crops. It has been suggested that co-ordinate control of multiple steps of the pathway with the help of regulatory genes would lead to a more predictable control of metabolic flux. Regulation of anthocyanin biosynthesis has been studied in a common ornamental plant, Gerbera hybrida (Asteraceae). An R2R3-type MYB factor, GMYB10, shares high sequence similarity and is phylogenetically grouped together with previously characterized regulators of anthocyanin pigmentation. Ectopic expression of GMYB10 leads to strongly enhanced accumulation of anthocyanin pigments as well as to an altered pigmentation pattern in transgenic gerbera plants. Anthocyanin analysis indicates that GMYB10 specifically induces cyanidin biosynthesis in undifferentiated callus and in vegetative tissues. Furthermore, in floral tissues enhanced pelargonidin production is detected. Microarray analysis using the gerbera 9K cDNA array revealed a highly predicted set of putative target genes for GMYB10 including new gene family members of both early and late biosynthetic genes of the flavonoid pathway. However, completely new candidate targets, such as a serine carboxypeptidase-like gene as well, as two new MYB domain factors, GMYB11 and GMYB12, whose exact function in phenylpropanoid biosynthesis is not clear yet, were also identified.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
7.4
作者：
Deluc, L;Barrieu, F;Hamdi, S
通讯作者：
Hamdi, S
影响因子：
7.2
作者：
Elomaa, P;Mehto, M;Teeri, TH
通讯作者：
Teeri, TH
影响因子：
5.1
作者：
HELARIUTTA, Y;ELOMAA, P;TEERI, TH
通讯作者：
TEERI, TH
影响因子：
7
作者：
Laitinen, RAE;Immanen, J;Elomaa, P
通讯作者：
Elomaa, P