Genetical control of 2D pattern and depth of the primordial furrow that prefigures 3D shape of the rhinoceros beetle horn.
Genetical control of 2D pattern and depth of the primordial furrow that prefigures 3D shape of the rhinoceros beetle horn.
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DOI：
10.1038/s41598-020-75709-y
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发表时间：
2020-10-29
影响因子：
4.6
通讯作者：
Gotoh H
中科院分区：
文献类型：
--
作者：
Adachi H;Matsuda K;Niimi T;Kondo S;Gotoh H
亚洲犀牛甲虫的头角发育为广泛折叠的原基，然后在蛹蜕皮时展开成最终的3D形状。甲虫角发育过程中原基的折叠模式预示了角最终三维结构的信息。然而，原基上皮折叠的发育机制尚不清楚。在这项研究中，我们解决了这个差距，我们的理解的三维角形状的甲虫的发展模式，通过专注于形成犁沟的表面的原基，成为分叉的3D形状的角。通过RNAi的基因敲除分析，我们发现Notch基因的敲除扰乱了整个角原始沟深，而不影响2D沟模式。相比之下，CyclinE的敲除改变了2D角原始犁沟模式，而不影响犁沟深度。我们的研究结果表明，在甲虫角的发展过程中，原始表面沟的深度和2D图案至少部分独立地受到调节，以及两者如何改变角的最终3D形状。
The head horn of the Asian rhinoceros beetle develops as an extensively folded primordium before unfurling into its final 3D shape at the pupal molt. The information of the final 3D structure of the beetle horn is prefigured in the folding pattern of the developing primordium. However, the developmental mechanism underlying epithelial folding of the primordium is unknown. In this study, we addressed this gap in our understanding of the developmental patterning of the 3D horn shape of beetles by focusing on the formation of furrows at the surface of the primordium that become the bifurcated 3D shape of the horn. By gene knockdown analysis via RNAi, we found that knockdown of the gene Notch disturbed overall horn primordial furrow depth without affecting the 2D furrow pattern. In contrast, knockdown of CyclinE altered 2D horn primordial furrow pattern without affecting furrow depth. Our results show how the depth and 2D pattern of primordial surface furrows are regulated at least partially independently during beetle horn development, and how both can alter the final 3D shape of the horn.
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影响因子：
4.6
作者：
Matsuda K;Gotoh H;Tajika Y;Sushida T;Aonuma H;Niimi T;Akiyama M;Inoue Y;Kondo S
通讯作者：
Kondo S
DOI：
10.1534/g3.111.001537
发表时间：
2012-02
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Angelini DR;Smith FW;Jockusch EL
通讯作者：
Jockusch EL
影响因子：
4.5
作者：
Morita, Shinichi;Ando, Toshiya;Niimi, Teruyuki
通讯作者：
Niimi, Teruyuki
影响因子：
3.8
作者：
Riddiford, LM;Hiruma, K;Nelson, CA
通讯作者：
Nelson, CA
DOI：
10.1073/pnas.0701209104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Emlen, Douglas J.;Lavine, Laura Corley;Ewen-Campen, Ben
通讯作者：
Ewen-Campen, Ben