Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5
Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5
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海洋光合细菌Rhodovulum sulfidophilum P5生产聚羟基丁酸酯
DOI：
10.1007/s00343-012-1132-9
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发表时间：
2012-07
影响因子：
--
通讯作者：
Wang Guangce
中科院分区：
文献类型：
--
作者：
Cai Jinling;Wei Ying;Zhao Yupeng;Pan Guanghua;Wang Guangce
在有氧暗条件下分批培养海洋光合细菌Rhodovulum sulfidophilum P5，研究了不同的NaCl浓度、氮源、碳源和碳氮摩尔比对生物量积累和聚羟基丁酸酯（PHB）产量的影响。结果表明，菌株P5中PHB的积累是一个与生长相关的过程。菌株 P5 在 2%–3% NaCl 时具有最大生物量和 PHB 积累，表明该细菌可以在天然海水盐度下维持生长并可能产生 PHB。氮源试验中，以蛋白胨和氯化铵为唯一氮源时，生物量积累最大（8.10±0.09 g/L），PHB产量最大（1.11±0.13 g/L，占细胞干重的14.62%±2.2）。 NH4+-N 比其他氮源更有利于 PHB 生产。碳源测试中，以苹果酸为唯一碳源时获得最大生物量浓度（7.65±0.05 g/L），以丙酮酸钠为唯一碳源时获得最大PHB产量（5.03±0.18 g/L，占细胞干重的66.93%±1.69%）。碳氮比测试中，分别选择丙酮酸钠和氯化铵作为碳源和氮源。生物量积累（8.77±0.58 g/L）和 PHB 生产（6.07±0.25 g/L 和细胞干重的 69.25%±2.05%）的最佳碳氮摩尔比为 25。结果为 R. sulfidophilum P5 生产 PHB 提供了有价值的数据，并且正在进一步研究最佳细胞生长和 PHB 产量。
The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate (PHB) production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%–3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation (8.10±0.09 g/L) and PHB production (1.11±0.13 g/L and 14.62%±2.2 of the cell dry weight) were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH4+-N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration (7.65±0.05 g/L) was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB (5.03±0.18 g/L and 66.93%±1.69% of the cell dry weight) was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation (8.77±0.58 g/L) and PHB production (6.07±0.25 g/L and 69.25%±2.05% of the cell dry weight) was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield.
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影响因子：
11.4
作者：
Mokhtari-Hosseini, Zahra B.;Vasheghani-Farahani, Ebrahim;Darani, Kianoush Khosravi
通讯作者：
Darani, Kianoush Khosravi
影响因子：
2.8
作者：
P. Weaver;J. Wall;H. Gest
通讯作者：
P. Weaver;J. Wall;H. Gest
影响因子：
11.4
作者：
Nath, A.;Dixit, M.;Desai, A. J.
通讯作者：
Desai, A. J.
影响因子：
6.7
作者：
Gouda, MK;Swellam, AE;Omar, SH
通讯作者：
Omar, SH
影响因子：
2.8
作者：
Karin Malmcrona-Friberg;A. Tunlid;P. Mårdén;S. Kjelleberg;G. Odham
通讯作者：
Karin Malmcrona-Friberg;A. Tunlid;P. Mårdén;S. Kjelleberg;G. Odham