Free access to a running-wheel advances the phase of behavioral and physiological circadian rhythms and peripheral molecular clocks in mice.
Free access to a running-wheel advances the phase of behavioral and physiological circadian rhythms and peripheral molecular clocks in mice.
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DOI：
10.1371/journal.pone.0116476
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Oishi K
中科院分区：
文献类型：
--
作者：
Yasumoto Y;Nakao R;Oishi K
动物的行为和生理昼夜节律是由内源性振荡器控制的。啮齿类动物自愿跑轮被认为是人类有氧运动的合适模式。我们通过分析进食的时间分布、核心体温、血浆激素浓度以及生物钟和生物钟控制基因的外周表达来评估慢性自愿运动对昼夜节律系统的影响，这些小鼠被安置在久坐（SED）条件下或自由进入跑步轮（RW）四周。自愿跑轮活动提前了小鼠体温、食物摄入量和皮质酮分泌增加的昼夜节律阶段。时钟和时钟控制基因的昼夜节律表达在RW小鼠中受到组织和基因特异性影响。e- box依赖性生物钟基因Per1、Per2、Nr1d1和Dbp的时间表达在肝脏和白色脂肪组织中略有提前，但在棕色脂肪组织和骨骼肌中没有。RW小鼠骨骼肌中Per1、Per2和Nr1d1的峰值表达水平显著升高。在SED和RW小鼠中，参与胆固醇和脂肪酸代谢的生物钟控制基因的昼夜节律阶段和肝脏mRNA表达水平显著不同。这些发现表明，内源性生物钟控制的自愿轮跑活动向系统地控制行为和生理节律的中央生物钟提供反馈。
Behavioral and physiological circadian rhythms are controlled by endogenous oscillators in animals. Voluntary wheel-running in rodents is thought to be an appropriate model of aerobic exercise in humans. We evaluated the effects of chronic voluntary exercise on the circadian system by analyzing temporal profiles of feeding, core body temperature, plasma hormone concentrations and peripheral expression of clock and clock-controlled genes in mice housed under sedentary (SED) conditions or given free access to a running-wheel (RW) for four weeks. Voluntary wheel-running activity advanced the circadian phases of increases in body temperature, food intake and corticosterone secretion in the mice. The circadian expression of clock and clock-controlled genes was tissue- and gene-specifically affected in the RW mice. The temporal expression of E-box-dependent circadian clock genes such as Per1, Per2, Nr1d1 and Dbp were slightly, but significantly phase-advanced in the liver and white adipose tissue, but not in brown adipose tissue and skeletal muscle. Peak levels of Per1, Per2 and Nr1d1 expression were significantly increased in the skeletal muscle of RW mice. The circadian phase and levels of hepatic mRNA expression of the clock-controlled genes that are involved in cholesterol and fatty acid metabolism significantly differed between SED and RW mice. These findings indicated that endogenous clock-governed voluntary wheel-running activity provides feedback to the central circadian clock that systemically governs behavioral and physiological rhythms.
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影响因子：
8.2
作者：
Novak, Colleen M.;Burghardt, Paul R.;Levine, James A.
通讯作者：
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影响因子：
4.6
作者：
Kuroda, Hiroaki;Tahara, Yu;Saito, Keisuke;Ohnishi, Nobuaki;Kubo, Yuji;Seo, Yasuhiro;Otsuka, Makiko;Fuse, Yuta;Ohura, Yuki;Hirao, Akiko;Shibata, Shigenobu
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Shibata, Shigenobu
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Marcheva, Biliana;Ramsey, Kathryn M;Peek, Clara B;Affinati, Alison;Maury, Eleonore;Bass, Joseph
通讯作者：
Bass, Joseph
影响因子：
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作者：
Leise, T. L.;Harrington, M. E.;Molyneux, P. C.;Song, I.;Queenan, H.;Zimmerman, E.;Lall, G. S.;Biello, S. M.
通讯作者：
Biello, S. M.
影响因子：
5.5
作者：
Mendoza, Jorge;Pevet, Paul;Challet, Etienne
通讯作者：
Challet, Etienne