Cocaine alters dendritic spine density in cortical and subcortical brain regions of the postpartum and virgin female rat.
Cocaine alters dendritic spine density in cortical and subcortical brain regions of the postpartum and virgin female rat.
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DOI：
10.1002/syn.20918
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发表时间：
2011-09
期刊：
影响因子：
2.3
通讯作者：
Luine, Victoria
中科院分区：
文献类型：
--
作者：
Frankfurt, Maya;Salas-Ramirez, Kaliris;Friedman, Eitan;Luine, Victoria
怀孕期间使用可卡因会导致母亲和后代严重的神经和行为缺陷。本研究旨在比较可卡因暴露对产后和处女雌性大鼠大脑脊柱密度的影响。从妊娠第 8-20 天开始，定时向怀孕初产大鼠注射可卡因 (30 mg/kg) 或生理盐水，每天一次。分娩后二十四小时，对母脑进行处理以进行高尔基体浸渍。由于可卡因对雌性大鼠的影响尚未确定，未交配的雌性大鼠也注射相同剂量的可卡因或生理盐水12天，并在最后一次注射后24小时处死以进行比较。与可卡因治疗无关的处女雌性相比，怀孕大鼠的内侧杏仁核（MeA）和内侧视前区（MPOA）的棘密度明显更高，而 CA1 的棘密度更低。可卡因显着增加了怀孕大鼠前额叶皮层锥体细胞顶端分支（PFC，15%）、CA1 顶端分支（13%）和基底分支（14.8%）以及 MeA 细胞（28%）的树突棘密度。在 MPOA 中，给予可卡因导致怀孕大鼠的树突棘密度下降（14%）。在处女体内，可卡因的影响较小，但确实增加了 CA1 神经元和 MeA 两个分支的树突棘密度。本研究首次证明怀孕和处女之间的脊柱密度不同，并且怀孕使大脑更容易受到可卡因的影响，这具有重要的临床意义。
Cocaine use during pregnancy induces profound neural and behavioral deficits in both mother and offspring. The present study was designed to compare the effects of cocaine exposure on spine density of postpartum and virgin female rat brains. Timed, pregnant, primiparous rats were injected with either cocaine (30 mg/kg) or saline, once daily, from gestational day 8–20. Twenty four hours after giving birth, dam brains were processed for Golgi-impregnation. Since cocaine effects in female rats have not been determined, virgin females were also injected with the same dose of cocaine or saline for 12 days and sacrificed 24h after the last injection for comparison. Pregnant rats had significantly greater spine density in the medial amygdala (MeA) and medial preoptic area (MPOA) and lower spine density in CA1 than virgin females independent of cocaine treatment. Cocaine significantly increased dendritic spine density on the apical branch of pyramidal cells in the prefrontal cortex (PFC, 15%), both apical (13%) and basal (14.8%) branches of CA1 and cells in the MeA (28%) of pregnant rats. In the MPOA, cocaine administration resulted in a decrease in dendritic spine density (14%) in pregnant rats. In virgin females, cocaine had fewer effects but did increase dendritic spine density on both branches of CA1 neurons and in the MeA. The present study is the first to demonstrate that spine density differs between pregnant and virgin females and that pregnancy makes the brain more vulnerable to cocaine, which has important clinical implications.
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