MEDUSA: A cloud-based tool for the analysis of X-ray diffuse scattering to obtain the bending modulus from oriented membrane stacks.

MEDUSA: A cloud-based tool for the analysis of X-ray diffuse scattering to obtain the bending modulus from oriented membrane stacks.
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DOI:
10.1371/journal.pcbi.1011749
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发表时间:
2024-01
影响因子:
4.3
通讯作者:
--
中科院分区:
生物学2区
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--
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细胞的一个重要机械性质是其膜弯曲模量κ。在这里,我们介绍MEDUSA(MEbrane Diffuse Scattering Analysis),这是一种基于云的分析工具,用于通过X射线漫散射分析确定弯曲模量κ。MEDUSA使用GPU(图形处理单元)加速硬件和并行算法在几秒钟内有效地运行计算。MEDUSA的图形用户界面允许用户上传从不同来源收集的二维数据,执行背景减除和失真校正,选择感兴趣区域,运行拟合程序并输出拟合参数,膜的弯曲模量κ和压缩模量B。
An important mechanical property of cells is their membrane bending modulus, κ. Here, we introduce MEDUSA (MEmbrane DiffUse Scattering Analysis), a cloud-based analysis tool to determine the bending modulus, κ, from the analysis of X-ray diffuse scattering. MEDUSA uses GPU (graphics processing unit) accelerated hardware and a parallelized algorithm to run the calculations efficiently in a few seconds. MEDUSA’s graphical user interface allows the user to upload 2-dimensional data collected from different sources, perform background subtraction and distortion corrections, select regions of interest, run the fitting procedure and output the fitted parameters, the membranes’ bending modulus κ, and compressional modulus B.
DOI: 10.3389/fphys.2022.953257
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