Role of miR-34c in the cognitive function of epileptic rats induced by pentylenetetrazol.
Role of miR-34c in the cognitive function of epileptic rats induced by pentylenetetrazol.
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miR-34c对戊四氮致癫痫大鼠认知功能的影响
DOI：
10.3892/mmr.2018.8441
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发表时间：
2018-03
影响因子：
3.4
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Huang Y;Liu X;Liao Y;Liao Y;Zou D;Wei X;Huang Q;Wu Y
研究表明，microRNA(MiR)-34c可能在啮齿动物和灵长类动物的认知功能中发挥作用。先前的研究表明，在戊四氮(PTZ)诱导的慢性癫痫大鼠记忆障碍中，miR-34c表达增加。然而，miR-34c对癫痫大鼠认知功能影响的机制尚不清楚。因此，本研究观察了经miR-34c治疗后重复注射PTZ诱发的颞叶癫痫大鼠认知功能的变化，并与对照组进行了比较。在Morris水迷宫实验中，除学习记忆功能减退外，阿戈米尔组miR-34c表达增加。免疫印迹法检测海马区谷氨酸受体离子型N-甲基-D-天冬氨酸(NMDA)2B(NR2B)、磷酸化(P)还原烟酰胺腺嘌呤二核苷酸磷酸依赖的双黄素氧化还原酶1(NR1)和p-谷氨酸受体1(GluR1)蛋白的表达。此外，miR-34c组NR2B、p-nr1和p-Glu r1表达下调，表明miR-34c可能通过调节与长时程增强相关的α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic和nmda受体，对癫痫发作的认知功能起负性作用。
Studies suggest that microRNA (miR)-34c may serve a role in cognitive function in rodent and primate groups. A previous study demonstrated an increase in miR-34c expression in chronic epileptic rats with memory disorders, induced by pentylenetetrazol (PTZ). However, the mechanism underlying the effects of miR-34c on cognitive function in epileptic rats remains unclear. Therefore, the present study investigated alterations in cognitive function in temporal lobe epileptic rats, induced by repeated injections of PTZ, following treatment with an miR-34c agomir compared with a scramble group. Increased expression of miR-34c was observed in the agomir group, in addition to an increased deficit in learning and memory function in the Morris water maze test. Glutamate receptor ionotropic N-methyl-D-aspartate (NMDA) 2B (NR2B), phosphorylated (p)-reduced nicotinamide-adenine dinucleotide phosphate-dependent diflavin oxidoreductase 1 (NR1) and p-glutamate receptor 1 (GluR1) protein expression was detected in the hippocampus using western blotting. Additionally, the downregulation of NR2B, p-NR1 and p-GluR1 in the miR-34c agomir group demonstrated that miR-34c may serve a negative role in cognitive function in epileptic seizures, by dysregulating NMDA and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, which are associated with long-term potentiation.
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影响因子：
2.5
作者：
Parsons, Michael J.;Grimm, Christina H.;Paya-Cano, Jose L.;Sugden, Karen;Nietfeld, Wilfried;Lehrach, Hans;Schalkwyk, Leonard C.
通讯作者：
Schalkwyk, Leonard C.
影响因子：
--
作者：
Ouellet DL;Perron MP;Gobeil LA;Plante P;Provost P
通讯作者：
Provost P
影响因子：
3.3
作者：
Pierrefiche O
通讯作者：
Pierrefiche O
影响因子：
3.9
作者：
Cao, Shu-e;Tian, Jianmin;Zhang, Yongqiang
通讯作者：
Zhang, Yongqiang
影响因子：
4.8
作者：
Ryan B;Joilin G;Williams JM
通讯作者：
Williams JM