High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application.
High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application.
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猪粪施用后土壤中可培养的四环素抗性细菌的多样性和丰富度
DOI：
10.1038/s41598-018-20050-8
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发表时间：
2018-01-24
影响因子：
4.6
通讯作者：
Hu J
中科院分区：
文献类型：
--
作者：
Kang Y;Li Q;Yin Z;Shen M;Zhao H;Bai Y;Mei L;Hu J
通过进行模拟施肥的微观实验，我们通过培养依赖性方法和 PCR 检测评估了猪粪 (PM) 到施肥土壤中四环素抗性细菌 (TRB) 和相应四环素抗性基因 (TRG) 的动态分布。 PM中可培养TRB含量最高，其次是施肥土壤和未施肥土壤。通过限制性片段长度多态性 (RFLP) 分析，TRB 分别归属于 PM、未施肥土壤和施肥土壤中的 29、20 和 153 个操作分类单位 (OTU)。经过鉴定，它们被进一步分为 19、12 和 62 个物种，显示施用 PM 后土壤中可培养 TRB 的多样性有所增强。与PM和未施肥土壤相比，施肥土壤中潜在致病性TRB的比例分别下降了69.35%和41.92%。蜡状芽孢杆菌可能是各种环境下广泛分布的TRB，红平红球菌和不动杆菌属。可能通过施肥从 PM 传播到土壤。同时，相对于PM，tetL是未施肥和施肥土壤中最常见的外排泵基因；tetB(P)和tet36在PM中常见，而astetO在未施肥和施肥土壤样品中占主导地位。测序表明，施肥土壤中随机选择的 TRB 中超过 65% 的获得性抗性源自 PM。
By performing a microcosm experiment mimicking fertilization, we assessed the dynamic distribution of tetracycline-resistant bacteria (TRB) and corresponding tetracycline resistance genes (TRGs) from pig manure (PM) to the fertilized soil, by culture-dependent methods and PCR detection. Cultivable TRB were most abundant in PM, followed by fertilized soil and unfertilized soil. By restriction fragment length polymorphism (RFLP) analysis, TRB were assigned to 29, 20, and 153 operational taxonomic units (OTUs) in PM, unfertilized soil, and fertilized soil, respectively. After identification, they were further grouped into 19, 12, and 62 species, showing an enhanced diversity of cultivable TRB in the soil following PM application. The proportions of potentially pathogenic TRB in fertilized soil decreased by 69.35% and 41.92% compared with PM and unfertilized soil.Bacillus cereuswas likely widely distributed TRB under various environments, andRhodococcus erythropolisandAcinetobactersp. probably spread from PM to the soil via fertilization. Meanwhile,tetL was the most common efflux pump gene in both unfertilized and fertilized soils relative to PM;tetB(P) andtet36 were common in PM, whereastetO was predominant in unfertilized and fertilized soil samples. Sequencing indicated that over 65% of randomly selected TRB in fertilized soil with acquired resistance derived from PM.
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影响因子：
1.2
作者：
Chen, Lei;Song, Yajing;Jin, Meilin
通讯作者：
Jin, Meilin
对大量人类肠道微生物群中抗生素抗性基因进行宏基因组分析
影响因子：
16.6
作者：
Hu, Yongfei;Yang, Xi;Zhu, Baoli
通讯作者：
Zhu, Baoli
影响因子：
4.4
作者：
Agerso, Y;Sandvang, D
通讯作者：
Sandvang, D
影响因子：
4.9
作者：
Abril, Carlos;Brodard, Isabelle;Perreten, Vincent
通讯作者：
Perreten, Vincent
DOI：
10.1073/pnas.0402414101
发表时间：
2004-06-01
影响因子：
11.1
作者：
Hoffmaster, AR;Ravel, J;Fraser, CM
通讯作者：
Fraser, CM