Human coronaviruses are uncommon in patients with gastrointestinal illness.
Human coronaviruses are uncommon in patients with gastrointestinal illness.
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DOI：
10.1016/j.jcv.2010.03.007
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发表时间：
2010-06
影响因子：
8.8
通讯作者：
Huang, Yung T.
中科院分区：
文献类型：
--
作者：
Esper, Frank;Ou, Zhen;Huang, Yung T.
冠状病毒感染许多动物物种，导致各种疾病，包括呼吸道、神经和肠道疾病。人类冠状病毒(HCoV)主要与呼吸道疾病有关，但也与肠道疾病有关。目的应用RT-PCR方法检测儿童和成人胃肠道疾病患者粪便标本中冠状病毒的感染情况。提交进行感染性腹泻检测的临床样本是从2007年12月至2008年3月收集的。使用针对HCoV-229E、HCoV-OC43、HCoV-NL63和HCoV-HKU1的引物集对粪便进行RNA提取和RT-PCR。对冠状病毒阳性样本的临床数据进行了回顾和记录。收集了479名患者的样本，其中包括151名儿童(≤；18岁)和328名成年人(>；18岁)。在这些样本中，4名患者(1.3%，2名成人，2名儿童)筛查出冠状病毒存在。所有检测到的冠状病毒均被鉴定为HCoV-HKU1。未检出HCoV-229E、HCoV-NL63或HCoV-OC43阳性粪便。所有HCoV-HKU1阳性样本都发生在1月中旬至2月中旬之间。HCoV-HKU1阳性患者的临床表现为腹泻、呕吐和呼吸道症状。3名患者(75%)入院，中位住院时间为6天。冠状病毒作为一组病毒，在出现胃肠道疾病的患者的粪便样本中并不常见。在患有胃肠道疾病的儿童和成人的粪便样本中可以发现hCoV-HKU1，大多数人也有呼吸道症状。没有粪便样本筛查出HCoV-NL63、HCoV-229E或HCoV-OC43呈阳性。
Coronaviruses infect numerous animal species causing a variety of illnesses including respiratory, neurologic and enteric disease. Human coronaviruses (HCoV) are mainly associated with respiratory tract disease but have been implicated in enteric disease. To investigate the frequency of coronaviruses in stool samples from children and adults with gastrointestinal illness by RT-PCR. Clinical samples submitted for infectious diarrhea testing were collected from December 2007 through March 2008. RNA extraction and RT-PCR was performed for stools negative for Clostridium difficile using primer sets against HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1. Clinical data from samples positive for coronaviruses were reviewed and recorded. Samples from 479 patients were collected including 151 pediatric (≤18 years), and 328 adults (>18 years). Of these samples, 4 patients (1.3%, 2 adult; 2 pediatric) screened positive for the presence of a coronavirus. All detected coronaviruses were identified as HCoV-HKU1. No stools screened positive for either HCoV-229E, HCoV-NL63 or HCoV-OC43. All HCoV-HKU1 positive samples occurred between mid-January to mid-February. Clinical manifestations from HCoV-HKU1 positive patients included diarrhea, emesis and respiratory complaints. Three (75%) patients were admitted to the hospital with a median length of stay of 6 days. Coronaviruses as a group are not commonly identified in stool samples of patients presenting with gastrointestinal illness. HCoV-HKU1 can be identified in stool samples from children and adults with gastrointestinal disease, with most individuals having respiratory findings as well. No stool samples screened positive for HCoV-NL63, HCoV-229E, or HCoV-OC43.
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影响因子：
9.4
作者：
Dijkman, Ronald;Jebbink, Maarten F.;van der Hoek, Lia
通讯作者：
van der Hoek, Lia
影响因子：
8.8
作者：
Shao, Xiuping;Guo, Xiaojie;Kahn, Jeffrey S.
通讯作者：
Kahn, Jeffrey S.
影响因子：
1.2
作者：
TSUNEMITSU, H;YONEMICHI, H;SHIMIZU, M
通讯作者：
SHIMIZU, M
影响因子：
8.8
作者：
Jin, Yu;Cheng, Wei-xia;Duan, Zhao-jun
通讯作者：
Duan, Zhao-jun
DOI：
10.1016/s0140-6736(04)16255-7
发表时间：
2004-05-22
期刊：
Lancet (London, England)
影响因子：
--
作者：
Cheng PK;Wong DA;Tong LK;Ip SM;Lo AC;Lau CS;Yeung EY;Lim WW
通讯作者：
Lim WW