Using Next-Generation Sequencing to Detect Differential Expression Genes in Bradysia odoriphaga after Exposure to Insecticides.
Using Next-Generation Sequencing to Detect Differential Expression Genes in Bradysia odoriphaga after Exposure to Insecticides.
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使用下一代测序检测接触杀虫剂后的差异表达基因
DOI：
10.3390/ijms18112445
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发表时间：
2017-11-17
影响因子：
5.6
通讯作者：
Su W
中科院分区：
文献类型：
--
作者：
Chen H;Lin L;Ali F;Xie M;Zhang G;Su W
韭菜迟眼蕈蚊（双翅目：眼蕈蚊科）是韭菜最重要的害虫。在中国，广泛且频繁地使用杀虫剂来防治韭菜迟眼蕈蚊。然而，杀虫剂毒死蜱和噻虫胺在防治韭菜迟眼蕈蚊方面的效果差异很大。利用新一代测序技术，与对照相比，在使用毒死蜱和噻虫胺处理6小时和48小时后，检测到韭菜迟眼蕈蚊中的差异表达基因（DEUs）。杀虫剂处理后，差异表达基因的数量在703到1161之间。在这些差异表达基因中，370 - 863个基因可归类为41 - 46类基因本体（GO），354 - 658个差异表达基因可映射到987 - 1623条京都基因与基因组百科全书（KEGG）通路。分析了与杀虫剂代谢相关基因的差异表达基因的表达情况。细胞色素P450样基因群是差异表达基因中最大的一组。在杀虫剂处理后，大多数谷胱甘肽S - 转移酶样基因下调，大多数钠通道样基因上调。最后，选择14个与杀虫剂代谢相关的基因，通过实时荧光定量聚合酶链反应（qRT - PCR）确定每种处理中的相对表达量。qRT - PCR和RNA测序（RNA - Seq）的结果相当吻合。我们的研究结果表明，新一代测序工具有助于鉴定与杀虫剂代谢相关的基因，并阐明毒死蜱和噻虫胺的杀虫机制。
Bradysia odoriphaga (Diptera: Sciaridae) is the most important pest of Chinese chive. Insecticides are used widely and frequently to control B. odoriphaga in China. However, the performance of the insecticides chlorpyrifos and clothianidin in controlling the Chinese chive maggot is quite different. Using next generation sequencing technology, different expression unigenes (DEUs) in B. odoriphaga were detected after treatment with chlorpyrifos and clothianidin for 6 and 48 h in comparison with control. The number of DEUs ranged between 703 and 1161 after insecticide treatment. In these DEUs, 370–863 unigenes can be classified into 41–46 categories of gene ontology (GO), and 354–658 DEUs can be mapped into 987–1623 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expressions of DEUs related to insecticide-metabolism-related genes were analyzed. The cytochrome P450-like unigene group was the largest group in DEUs. Most glutathione S-transferase-like unigenes were down-regulated and most sodium channel-like unigenes were up-regulated after insecticide treatment. Finally, 14 insecticide-metabolism-related unigenes were chosen to confirm the relative expression in each treatment by quantitative Real Time Polymerase Chain Reaction (qRT-PCR). The results of qRT-PCR and RNA Sequencing (RNA-Seq) are fairly well-established. Our results demonstrate that a next-generation sequencing tool facilitates the identification of insecticide-metabolism-related genes and the illustration of the insecticide mechanisms of chlorpyrifos and clothianidin.
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DOI：
10.1073/pnas.0606146103
发表时间：
2006-12-12
影响因子：
11.1
作者：
Borovsky, Dov;Rabindran, Shailaja;DeLoof, Arnold
通讯作者：
DeLoof, Arnold
影响因子：
3.7
作者：
Chen H;Lin L;Xie M;Zhang G;Su W
通讯作者：
Su W
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
2
作者：
Fang, Fujin;Wang, Weijie;Zhu, Changliang
通讯作者：
Zhu, Changliang
影响因子：
3.8
作者：
Kostaropoulos, I;Papadopoulos, AI;Papadopoulou-Mourkidou, E
通讯作者：
Papadopoulou-Mourkidou, E