Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii
Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii
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从蓖麻油中分离的化合物的细胞毒性作用
DOI：
10.3390/molecules24010145
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发表时间：
2019-01
期刊：
影响因子：
4.6
通讯作者：
Iweala Emeka J
中科院分区：
文献类型：
--
作者：
Yakubu Omolara F;Adebayo Abiodun H;Dokunmu Titilope M;Zhang Ying-Jun;Iweala Emeka J
本研究旨在探讨从蓖麻中分离得到的生物活性化合物对肿瘤细胞株的体外抗癌作用。用乙醇提取植物叶片，再用石油醚、乙酸乙酯和正丁醇依次进行分离。采用薄层色谱（TLC）和柱层析（CC）对乙酸乙酯部分进行了植物化学研究。通过质谱、1H-NMR和13C-NMR分析对从乙酸乙酯部分得到的纯化合物进行了结构解析。随后用5种不同的癌细胞系HL-60、SMMC-7721、A-549、MCF-7、SW-480和正常肺上皮细胞系BEAS-2B筛选分离的化合物，以评估其细胞毒性作用。分离得到的9个化合物分别为没食子酸、没食子酸乙酯、毛丝蛋白、槲皮素-3- o -鼠李糖苷、杨梅素-3- o -鼠李糖苷、1,4,6-三- o -没食子酰葡萄糖、3,4,6-三- o -没食子酰葡萄糖、1,2,6-三- o -没食子酰葡萄糖和4,6-二- o -没食子酰葡萄糖。Corilagin对MCF-7细胞的IC50值为33.18 μg/mL，与顺铂的IC50值为27.43 μg/mL相当。本研究结果提示，从毛茛中分离得到的毛茛蛋白具有开发作为抗乳腺癌细胞生长的有效药物的潜力。
This study was designed to explore the in vitro anticancer effects of the bioactive compounds isolated from Ricinodendron heudelotii on selected cancer cell lines. The leaves of the plant were extracted with ethanol and partitioned in sequence with petroleum ether, ethyl acetate, and n-butanol. The ethyl acetate fraction was phytochemically studied using thin layer chromatography (TLC) and column chromatography (CC). Structural elucidation of pure compounds obtained from the ethyl acetate fraction was done using mass spectra, 1H-NMR, and 13C-NMR analysis. The isolated compounds were subsequently screened using five different cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7, SW-480, and normal lung epithelial cell line, BEAS-2B, to assess their cytotoxic effects. Nine compounds were isolated and structurally elucidated as gallic acid, gallic acid ethyl ester, corilagin, quercetin-3-O-rhamnoside, myricetin-3-O-rhamnoside, 1,4,6-tri-O-galloyl glucose, 3,4,6-tri-O-galloyl glucose, 1,2,6-tri-O-galloyl glucose, and 4,6-di-O-galloyl glucose. Corilagin exhibited the most cytotoxic activity with an IC50 value of 33.18 μg/mL against MCF-7 cells, which were comparable to cisplatin with an IC50 value of 27.43 µg/mL. The result suggests that corilagin isolated from R. heudelotii has the potential to be developed as an effective therapeutic agent against the growth of breast cancer cells.
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影响因子：
6.4
作者：
Sylla, Bakary S.;Wild, Christopher P.
通讯作者：
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作者：
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通讯作者：
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影响因子：
0.5
作者：
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