Transferrin, insulin, and progesterone modulate intracellular concentrations of coenzyme Q and cholesterol, products of the mevalonate pathway, in undifferentiated PC12 cells.
Transferrin, insulin, and progesterone modulate intracellular concentrations of coenzyme Q and cholesterol, products of the mevalonate pathway, in undifferentiated PC12 cells.
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在未分化的PC12细胞中，转铁蛋白、胰岛素和黄体酮调节细胞内辅酶Q和胆固醇的浓度，这些都是甲氧戊酸途径的产物。
DOI：
10.3164/jcbn.22-115
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发表时间：
2023-05
影响因子：
2.4
通讯作者：
中科院分区：
文献类型：
--
作者：
辅酶 Q (CoQ) 不仅作为线粒体电子传递系统的必需脂质，而且作为抗氧化剂也很重要。 CoQ 水平会随着衰老和各种疾病的发生而降低。口服辅酶Q不容易被大脑吸收，因此有必要开发一种方法来增加神经元中辅酶Q的量。辅酶 Q 与胆固醇一样，通过甲羟戊酸途径合成。转铁蛋白、胰岛素和黄体酮是神经元培养中使用的因子。在这项研究中，我们确定了这些试剂对细胞 CoQ 和胆固醇水平的影响。转铁蛋白、胰岛素和黄体酮的施用增加了未分化 PC12 细胞中的细胞 CoQ 水平。当去除血清并仅施用胰岛素时，细胞内 CoQ 水平增加。当同时施用转铁蛋白、胰岛素和黄体酮时，这种增加甚至更加明显。服用转铁蛋白、胰岛素和黄体酮可降低胆固醇水平。黄体酮治疗以浓度依赖性方式降低细胞内胆固醇水平。我们的研究结果表明，转铁蛋白、胰岛素和黄体酮可能有助于调节辅酶 Q 水平和胆固醇水平，这些都是甲羟戊酸途径的产物。
Coenzyme Q (CoQ) is important not only as an essential lipid for the mitochondrial electron transport system, but also as an antioxidant. CoQ levels decrease during aging and in various diseases. Orally administered CoQ is not readily taken up in the brain, so it is necessary to develop a method to increase the amount of CoQ in neurons. CoQ is synthesized via mevalonate pathway, like cholesterol. Transferrin, insulin, and progesterone are factors used in the culture of neurons. In this study, we determined the effect of these reagents on cellular CoQ and cholesterol levels. The administration of transferrin, insulin, and progesterone increased cellular CoQ levels in undifferentiated PC12 cells. When serum was removed and only insulin was administered, intracellular CoQ levels increased. This increase was even more pronounced with concurrent administration of transferrin, insulin, and progesterone. Cholesterol level decreased by the administration of transferrin, insulin, and progesterone. Progesterone treatment lowered intracellular cholesterol levels in a concentration-dependent manner. Our findings suggest that transferrin, insulin, and progesterone may be useful in regulating CoQ levels and cholesterol levels, which are products of the mevalonate pathway.
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DOI：
10.1016/0005-2728(68)90214-4
发表时间：
1968-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
AITHAL, HN;JOSHI, VC;RAMASARMA, T
通讯作者：
RAMASARMA, T
影响因子：
1.9
作者：
KALEN, A;APPELKVIST, EL;DALLNER, G
通讯作者：
DALLNER, G
影响因子：
4.8
作者：
Metherall, JE;Li, HJ;Waugh, K
通讯作者：
Waugh, K
DOI：
10.1073/pnas.76.1.514
发表时间：
1979-01-01
影响因子：
11.1
作者：
BOTTENSTEIN, JE;SATO, GH
通讯作者：
SATO, GH
影响因子：
3.3
作者：
GOHIL, K;ROTHFUSS, L;PACKER, L
通讯作者：
PACKER, L