Unveiling of brain transcriptome of masked palm civet (Paguma larvata) with chronic infection of Toxoplasma gondii.
Unveiling of brain transcriptome of masked palm civet (Paguma larvata) with chronic infection of Toxoplasma gondii.
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DOI：
10.1186/s13071-022-05378-5
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发表时间：
2022-07-24
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究的目的是了解野生动物感染弓形虫后的转录组变化。果子狸是一种人工驯养的动物，被用作野生物种的模型。转录组分析被用来研究驯化的果子狸在慢性感染T。刚地。用105 T.感染4个月后收集弓形虫包囊及其脑组织。RNA测序（RNA-Seq）用于深入了解因感染而差异表达的基因谱。还使用定量逆转录PCR（qRT-PCR）来验证通过测序获得的一组差异表达基因（DEG）的表达水平。从测序结果中筛选DEG并进行分析。共检测到2808个DEG，其中860个上调，1948个下调。通过qRT-PCR确认RNA-Seq结果。基于基因本体富集分析，DEG主要在细胞过程和代谢过程中富集。京都基因百科全书和基因组通路分析表明，感染后果子狸脑内的转录变化随感染过程而演变，DEG主要富集在信号转导、免疫系统过程、运输和分解代谢通路中。最后，从免疫信号通路中鉴定出10个必需的驱动基因。本研究揭示了新的宿主基因，为T.野生动物的弓形虫感染在线版本包含补充材料，可通过10.1186/s13071-022-05378-5获得。
The aim of this study was to gain an understanding of the transcriptomic changes that occur in a wild species when infected with Toxoplasma gondii. The masked palm civet, an artifically domesticated animal, was used as the model of a wild species. Transcriptome analysis was used to study alterations in gene expression in the domesticated masked palm civet after chronic infection with T. gondii. Masked palm civets were infected with 105 T. gondii cysts and their brain tissue collected after 4 months of infection. RNA sequencing (RNA-Seq) was used to gain insight into the spectrum of genes that were differentially expressed due to infection. Quantitative reverse-transcription PCR (qRT-PCR) was also used to validate the level of expression of a set of differentially expressed genes (DEGs) obtained by sequencing. DEGs were screened from the sequencing results and analyzed. A total of 2808 DEGs were detected, of which 860 were upregulated and 1948 were downregulated. RNA-Seq results were confirmed by qRT-PCR. DEGs were mainly enriched in cellular process and metabolic process based on gene ontology enrichment analysis. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that transcriptional changes in the brain of infected masked palm civets evolved over the course of infection and that DEGs were mainly enriched in the signal transduction, immune system processes, transport and catabolic pathways. Finally, 10 essential driving genes were identified from the immune signaling pathway. This study revealed novel host genes which may provide target genes for the development of new therapeutics and detection methods for T. gondii infection in wild animals. The online version contains supplementary material available at 10.1186/s13071-022-05378-5.
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影响因子：
4.6
作者：
Hatam-Nahavandi K;Calero-Bernal R;Rahimi MT;Pagheh AS;Zarean M;Dezhkam A;Ahmadpour E
通讯作者：
Ahmadpour E
影响因子：
3.2
作者：
Liu Q;Wang ZD;Huang SY;Zhu XQ
通讯作者：
Zhu XQ
影响因子：
3.2
作者：
Jia B;Lu H;Liu Q;Yin J;Jiang N;Chen Q
通讯作者：
Chen Q
DOI：
10.4269/ajtmh.2011.10-0718
发表时间：
2011-05-01
影响因子：
3.3
作者：
Mayor, Angel M.;Santos, Diana M. Fernandez;Hunter-Mellado, Robert F.
通讯作者：
Hunter-Mellado, Robert F.
猫的急性弓形虫感染诱导由免疫特征主导的组织特异性转录反应
影响因子：
7.3
作者：
Cong W;Dottorini T;Khan F;Emes RD;Zhang FK;Zhou CX;He JJ;Zhang XX;Elsheikha HM;Zhu XQ
通讯作者：
Zhu XQ