Diagnosis of toxoplasmosis and typing of Toxoplasma gondii.
Diagnosis of toxoplasmosis and typing of Toxoplasma gondii.
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弓形虫病的诊断和弓形虫分型
DOI：
10.1186/s13071-015-0902-6
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发表时间：
2015-05-28
影响因子：
3.2
通讯作者：
Zhu XQ
中科院分区：
文献类型：
--
作者：
Liu Q;Wang ZD;Huang SY;Zhu XQ
弓形虫病是由专性细胞内原生动物弓形虫引起的一种重要的人畜共患病，具有重要的医学和兽医意义。这种疾病主要是通过摄入含有活组织囊的未煮熟或生肉，或通过摄入被卵囊污染的食物或水而感染的。弓形虫感染的诊断和基因特征对于弓形虫病的监测、预防和控制至关重要。传统的弓形虫病诊断方法包括病原学、免疫学和影像学技术。随着扩增寄生虫核酸的分子技术的出现，弓形虫病的诊断得到了改进。其中，基于聚合酶链式反应(PCR)的分子技术对弓形虫的遗传特征研究是有用的。基于多态多肽的血清分型方法有可能成为人和动物弓形虫分型的选择。本文综述了传统的非基于DNA的诊断方法，以及基于DNA的弓形虫分子诊断技术和基因特征。这些技术为进一步开发更有效、更准确地检测弓形虫感染奠定了基础。这些进展将有助于更好地了解弓形虫病的流行病学、预防和控制。
Toxoplasmosis, caused by the obligate intracellular protozoan Toxoplasma gondii, is an important zoonosis with medical and veterinary importance worldwide. The disease is mainly contracted by ingesting undercooked or raw meat containing viable tissue cysts, or by ingesting food or water contaminated with oocysts. The diagnosis and genetic characterization of T. gondii infection is crucial for the surveillance, prevention and control of toxoplasmosis. Traditional approaches for the diagnosis of toxoplasmosis include etiological, immunological and imaging techniques. Diagnosis of toxoplasmosis has been improved by the emergence of molecular technologies to amplify parasite nucleic acids. Among these, polymerase chain reaction (PCR)-based molecular techniques have been useful for the genetic characterization of T. gondii. Serotyping methods based on polymorphic polypeptides have the potential to become the choice for typing T. gondii in humans and animals. In this review, we summarize conventional non-DNA-based diagnostic methods, and the DNA-based molecular techniques for the diagnosis and genetic characterization of T. gondii. These techniques have provided foundations for further development of more effective and accurate detection of T. gondii infection. These advances will contribute to an improved understanding of the epidemiology, prevention and control of toxoplasmosis.
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影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
DOI：
10.1051/parasite/2013017
发表时间：
2013
期刊：
Parasite (Paris, France)
影响因子：
--
作者：
Ali-Heydari S;Keshavarz H;Shojaee S;Mohebali M
通讯作者：
Mohebali M
影响因子：
6.4
作者：
Ajzenberg, D;Cogné, N;Dardé, ML
通讯作者：
Dardé, ML
影响因子：
3.4
作者：
BALFOUR, AH;BRIDGES, JB;HARFORD, JP
通讯作者：
HARFORD, JP
影响因子：
2.1
作者：
BOBIC, B;SIBALIC, D;DJURKOVICDJAKOVIC, O
通讯作者：
DJURKOVICDJAKOVIC, O