Effect of ammonium and high light intensity on the accumulation of lipids in Nannochloropsis oceanica (CCAP 849/10) and Phaeodactylum tricornutum (CCAP 1055/1).
Effect of ammonium and high light intensity on the accumulation of lipids in Nannochloropsis oceanica (CCAP 849/10) and Phaeodactylum tricornutum (CCAP 1055/1).
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DOI：
10.1186/s13068-018-1061-8
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发表时间：
2018
影响因子：
6.3
通讯作者：
Vaidyanathan S
中科院分区：
文献类型：
--
作者：
Huete-Ortega M;Okurowska K;Kapoore RV;Johnson MP;Gilmour DJ;Vaidyanathan S
当微藻暴露在营养限制等应激条件下时，会积累脂质，可用于生产生物燃料。氮限制或剥夺是广泛采用的引起这种反应的策略。然而，这种策略与微藻生长的减少有关，导致总体脂质生产率较差。在这里，我们研究了减少氮源（铵）和过饱和光强度对两种模型但不同的微藻物种（三角褐指藻和微拟球藻）生长和诱导脂质积累的综合影响。我们假设，同化铵的能量成本较低，使生物体能够使用更多的还原剂进行脂质生物合成，而不会影响生长，并且强光（1000 µmol m−2 s−1）胁迫的影响将进一步刺激这种情况。我们研究了这两个物种在含有硝酸盐或铵作为氮源的培养基以及含有铵并用钨代替钼的附加培养基中生长时的生长和生理变化，并将其与不含氮的培养基中的生长进行了比较。我们的研究重点是暴露于治疗的早期阶段，以对应与诱导这两个物种中脂质积累相关的事件。在超饱和光强度下，与无氮培养基相比，在铵中生长时，三角角藻的脂质生产率增加了一倍，当培养基中存在钨代替钼时，脂质生产率进一步增加。相反，高光强度不会影响北海藻的生长和生理机能，并且铵的使用对脂质生产力有负面影响，当存在钨时，这种影响甚至更加明显。虽然在三角角藻中使用铵和超饱和光强度被认为是增加脂质生物合成的良好策略，但在这些条件下，没有观察到大洋藻的脂质生产力发生变化。这两项结果为更好地设计微藻生产生物燃料的工艺提供了相关方向，通过操纵生长条件而无需对微藻进行基因工程操作。
Microalgae accumulate lipids when exposed to stressful conditions such as nutrient limitation that can be used to generate biofuels. Nitrogen limitation or deprivation is a strategy widely employed to elicit this response. However, this strategy is associated with a reduction in the microalgal growth, leading to overall poor lipid productivities. Here, we investigated the combined effect of a reduced source of nitrogen (ammonium) and super-saturating light intensities on the growth and induction of lipid accumulation in two model but diverse microalgal species, Phaeodactylum tricornutum and Nannochloropsis oceanica. We hypothesized that the lower energy cost of assimilating ammonium would allow the organisms to use more reductant power for lipid biosynthesis without compromising growth and that this would be further stimulated by the effect of high light (1000 µmol m−2 s−1) stress. We studied the changes in growth and physiology of both species when grown in culture media that either contained nitrate or ammonium as the nitrogen source, and an additional medium that contained ammonium with tungsten in place of molybdenum and compared this with growth in media without nitrogen. We focused our investigation on the early stages of exposure to the treatments to correspond to events relevant to induction of lipid accumulation in these two species. At super-saturating light intensities, lipid productivity in P. tricornutum increased twofold when grown in ammonium compared to nitrogen free medium that increased further when tungsten was present in the medium in place of molybdenum. Conversely, N. oceanica growth and physiology was not compromised by the high light intensities used, and the use of ammonium had a negative effect on the lipid productivity, which was even more marked when tungsten was present. Whilst the use of ammonium and super-saturating light intensities in P. tricornutum was revealed to be a good strategy for increasing lipid biosynthesis, no changes in the lipid productivity of N. oceanica were observed, under these conditions. Both results provide relevant direction for the better design of processes to produce biofuels in microalgae by manipulating growth conditions without the need to subject them to genetic engineering manipulation.
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影响因子：
3.3
作者：
Huang, Xuxiong;Huang, Zhengzheng;Yan, Jiaqi
通讯作者：
Yan, Jiaqi
DOI：
10.1016/j.cep.2009.03.006
发表时间：
2009-06-01
影响因子：
4.3
作者：
Converti, Attilio;Casazza, Alessandro A.;Del Borghi, Marco
通讯作者：
Del Borghi, Marco
影响因子：
7.4
作者：
BROWN, JS
通讯作者：
BROWN, JS
影响因子：
7.4
作者：
Juergens, Matthew T.;Deshpande, Rahul R.;Shachar-Hill, Yair
通讯作者：
Shachar-Hill, Yair
DOI：
10.2174/2211550105666160322234434
发表时间：
2016-11
期刊：
Current biotechnology
影响因子：
--
作者：
Hounslow E;Kapoore RV;Vaidyanathan S;Gilmour DJ;Wright PC
通讯作者：
Wright PC