The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization.
The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization.
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DOI：
10.3390/polym13152473
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发表时间：
2021-07-27
期刊：
影响因子：
5
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Peng X;Han S;Wang K;Ding L;Liu Z;Zhang L
在这项研究中，釉原蛋白衍生的肽，TVH-19，这已被证实促进矿化，进行了评估，以获得其潜在的诱导牙本质小管闭塞。用激光共聚焦显微镜观察异硫氰酸荧光素（FITC）标记的TVH-19与脱矿牙本质表面的结合能力。此外，通过体外脱矿牙本质的再矿化研究了多肽的封闭功能。吸附结果表明，TVH-19可与羟基磷灰石和脱矿牙本质表面结合，尤其是牙周牙本质。扫描电子显微镜分析进一步揭示TVH-19产生矿物沉淀。TVH-19组的封堵率高于PBS组。此外，能量色散X射线光谱（EDX）的结果表明，由TVH-19诱导的新矿物的钙/磷（Ca/P）比接近的羟基磷灰石。衰减全内反射-傅里叶变换红外（ATR-FTIR）光谱和X射线衍射（XRD）结果表明，矿化后羟基磷灰石晶体的生长轴向延长，与天然牙本质成分相似。这些发现表明，TVH-19可以通过与牙周牙本质结合、促进矿物质沉积和减少牙本质小管之间的空间来有效地促进牙本质封闭。
In this study, the amelogenin-derived peptide, TVH-19, which has been confirmed to promote mineralization, was evaluated to derive its potential to induce dentinal tubule occlusion. The binding capability of fluorescein isothiocyanate (FITC)-labeled TVH-19 to the demineralized dentin surface was analyzed by confocal laser scanning microscopy (CLSM). Additionally, the sealing function of the peptide was studied through the remineralization of demineralized dentin in vitro. The adsorption results showed that TVH-19 could bind to the hydroxyapatite and demineralized dentin surfaces, especially to periodontal dentin. Scanning electron microscopy analysis further revealed that TVH-19 created mineral precipitates. The plugging rate in the TVH-19 group was higher than that in the PBS group. Moreover, energy-dispersive X-ray spectroscopy (EDX) results indicated that the calcium/phosphorus (Ca/P) ratio of the new minerals induced by TVH-19 was close to that of the hydroxyapatite. Attenuated total internal reflection-Fourier transform infrared (ATR-FTIR) spectrometry and X-ray diffraction (XRD) results indicated that the hydroxyapatite crystals formed via remineralization elongated the axial growth and closely resembled the natural dentin components. These findings indicate that TVH-19 can effectively promote dentin sealing by binding to the periodontal dentin, promoting mineral deposition, and reducing the space between the dentin tubules.
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影响因子：
3
作者：
Beniash, Elia;Metzler, Rebecca A.;Lam, Raymond S. K.;Gilbert, P. U. P. A.
通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
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DOI：
10.1016/j.bbrc.2020.10.095
发表时间：
2021-01-01
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
7
作者：
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通讯作者：
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影响因子：
1.5
作者：
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