Comparative venom gland transcriptome surveys of the saw-scaled vipers (Viperidae: Echis) reveal substantial intra-family gene diversity and novel venom transcripts.
Comparative venom gland transcriptome surveys of the saw-scaled vipers (Viperidae: Echis) reveal substantial intra-family gene diversity and novel venom transcripts.
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比较毒腺的比较转录组调查（Viperidae：echis）显示了家族内基因多样性和新型毒液转录本。
DOI：
10.1186/1471-2164-10-564
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发表时间：
2009-11-30
期刊：
影响因子：
4.4
通讯作者：
Wagstaff SC
中科院分区：
文献类型：
--
作者：
Casewell NR;Harrison RA;Wüster W;Wagstaff SC
蛇毒变异发生在所有分类学水平，并可能对蛇咬伤后的临床表现和抗蛇毒血清治疗效果产生重大影响。蛇毒成分的变化被认为是由于对特定饮食的适应而受到强烈的自然选择的影响。医学上重要的Echis属的成员在毒液成分上表现出相当大的变化，这已被证明与饮食的进化变化共同进化。我们采用毒腺转录组的方法，以调查毒素的多样性，在属和阐明的机制，导致猎物特异性的适应毒液组成。建立了E. E. leakeyi、E. coloratus和E.通过对来自毒腺cDNA文库的~1000个表达序列标签进行测序，一个标准化的方法允许一个全面的属内比较的毒腺配置文件进行，包括以前描述的E。单眼转录组。Blast注释揭示了蛇毒金属蛋白酶、C型凝集素、II组磷脂酶A2、丝氨酸蛋白酶、L-氨基氧化酶和生长因子在整个属的所有转录组中的存在。从至少一个，但不是所有的物种中获得编码去整合素，富含半胱氨酸的分泌蛋白和透明质酸酶的转录本。从大肠杆菌中鉴定出一组与溶酶体酸性脂肪酶具有相似性的新型毒液转录物。coloratus转录组，同时从E. p. leakeyi和E. coloratus分别。Echis毒液腺转录组的比较揭示了大量的属内毒液变异的代表性和最丰富的毒液毒素家族的簇数。已建立的毒素组的表达谱表现出与毒液相关的适应饮食描述从这个属没有明显的关联。因此，我们建议，主要毒液蛋白家族内的异构体多样性或转录表达水平的改变可能是负责猎物的特异性，而不是整个毒素家族的代表性或新的毒素家族的招聘的差异，虽然不能排除作为对脊椎动物喂养的反应的溶酶体酸性脂肪酶的招聘。在医学上重要的Echis属内明显的属内毒液变异的证据强烈主张进一步研究该属毒液变异的医学意义及其对抗蛇毒血清治疗的影响。
Venom variation occurs at all taxonomical levels and can impact significantly upon the clinical manifestations and efficacy of antivenom therapy following snakebite. Variation in snake venom composition is thought to be subject to strong natural selection as a result of adaptation towards specific diets. Members of the medically important genus Echis exhibit considerable variation in venom composition, which has been demonstrated to co-evolve with evolutionary shifts in diet. We adopt a venom gland transcriptome approach in order to investigate the diversity of toxins in the genus and elucidate the mechanisms which result in prey-specific adaptations of venom composition. Venom gland transcriptomes were created for E. pyramidum leakeyi, E. coloratus and E. carinatus sochureki by sequencing ~1000 expressed sequence tags from venom gland cDNA libraries. A standardised methodology allowed a comprehensive intra-genus comparison of the venom gland profiles to be undertaken, including the previously described E. ocellatus transcriptome. Blast annotation revealed the presence of snake venom metalloproteinases, C-type lectins, group II phopholipases A2, serine proteases, L-amino oxidases and growth factors in all transcriptomes throughout the genus. Transcripts encoding disintegrins, cysteine-rich secretory proteins and hyaluronidases were obtained from at least one, but not all, species. A representative group of novel venom transcripts exhibiting similarity to lysosomal acid lipase were identified from the E. coloratus transcriptome, whilst novel metallopeptidases exhibiting similarity to neprilysin and dipeptidyl peptidase III were identified from E. p. leakeyi and E. coloratus respectively. The comparison of Echis venom gland transcriptomes revealed substantial intrageneric venom variation in representations and cluster numbers of the most abundant venom toxin families. The expression profiles of established toxin groups exhibit little obvious association with venom-related adaptations to diet described from this genus. We suggest therefore that alterations in isoform diversity or transcript expression levels within the major venom protein families are likely to be responsible for prey specificity, rather than differences in the representation of entire toxin families or the recruitment of novel toxin families, although the recruitment of lysosomal acid lipase as a response to vertebrate feeding cannot be excluded. Evidence of marked intrageneric venom variation within the medically important genus Echis strongly advocates further investigations into the medical significance of venom variation in this genus and its impact upon antivenom therapy.
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影响因子：
3.3
作者：
Fürstenau, CR;Trentin, DD;Sarkis, JJF
通讯作者：
Sarkis, JJF
影响因子：
3.5
作者：
Deshimaru, M;Ogawa, T;Ohno, M
通讯作者：
Ohno, M
影响因子：
2.8
作者：
Fox, JW;Serrano, SMT
通讯作者：
Serrano, SMT
DOI：
10.1134/s1990747808010030
发表时间：
2008-03-01
影响因子：
0.5
作者：
Gorbacheva, E. V.;Starkov, V. G.;Vulfius, C. A.
通讯作者：
Vulfius, C. A.
影响因子：
2.8
作者：
Calvete, JJ;Marcinkiewicz, C;Sanz, L
通讯作者：
Sanz, L