Dynamic Changes of Pectin Epitopes in Cell Walls during the Development of the Procambium-Cambium Continuum in Poplar.
Dynamic Changes of Pectin Epitopes in Cell Walls during the Development of the Procambium-Cambium Continuum in Poplar.
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杨树原形成层-形成层连续体发育过程中细胞壁果胶表位的动态变化
DOI：
10.3390/ijms18081716
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发表时间：
2017-08-06
影响因子：
5.6
通讯作者：
He XQ
中科院分区：
文献类型：
--
作者：
Liu J;Hou J;Chen H;Pei K;Li Y;He XQ
利用免疫定位技术研究了杨树原形成层-形成层连续发育过程中果胶表位的变化。单克隆抗体JIM5标记低酯化程度的均半乳糖酸（HGA），单克隆抗体JIM7标记高甲基酯化程度的HGA。Arabinan和低酯化程度的HGA位于原形成层细胞壁，而低酯化程度的HGA位于原形成层细胞的切向壁，而半乳同时位于原形成层细胞的切向壁和径向壁，而几乎没有Arabinan位于形成层细胞的切向壁。果胶分布的变化发生在原形成层中出现周裂时。果胶表位在原形成层和形成层之间的分布也存在差异。阿拉伯多糖存在于初生木质部的所有细胞壁中，而不存在于次生木质部细胞的切向细胞壁中。半乳细胞仅存在于成熟的初生韧皮部中。此外，通过RNA测序鉴定出19个果胶甲基酯酶（PMEs）基因，其中6个基因表现出高度差异，可能参与了细胞壁酯化过程。研究结果为杨树原形成层-形成层连续体发育过程中果胶表位的动态变化提供了直接证据。
The change of pectin epitopes during procambium–cambium continuum development was investigated by immunolocalization in poplar. The monoclonal antibody JIM5 labels homogalacturonan (HGA) with a low degree of esterification, and the monoclonal antibody JIM7 labels HGA with a high degree of methyl-esterification. Arabinan, rather than galactan, and HGA with low degree of esterification were located in the cell walls of procambial, while HGA with a low degree of esterification was located in the tangential walls, and galactan was located in both the tangential and radial walls of procambial, yet nearly no arabinan was located in the tangential walls of the cambial cells. The changes in pectin distribution took place when periclinal divisions appeared within a procambial trace. The distribution difference of pectin epitopes was also present in procambium–cambium derivatives. The arabinan existed in all cell walls of primary xylem, but was absent from the tangential walls of secondary xylem cells. The galactan existed only in mature primary phloem. Furthermore, 19 pectin methylesterases (PMEs) genes were identified by RNA sequencing, six genes presented highly differentially and were supposed to be involved in the cell wall esterification process. The results provide direct evidence of the dynamic changes of pectin epitopes during the development of the procambium–cambium continuum in poplar.
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影响因子：
4
作者：
Arend, Matthias
通讯作者：
Arend, Matthias
影响因子：
4.3
作者：
Louvet, Romain;Cavel, Emilie;Pelloux, Jerome
通讯作者：
Pelloux, Jerome
影响因子：
7.4
作者：
Nishikubo, Nobuyuki;Takahashi, Junko;Mellerowicz, Ewa J.
通讯作者：
Mellerowicz, Ewa J.
影响因子：
6.9
作者：
Chateigner-Boutin AL;Suliman M;Bouchet B;Alvarado C;Lollier V;Rogniaux H;Guillon F;Larré C
通讯作者：
Larré C
影响因子：
6.5
作者：
Guzman, Paula;Fernandez, Victoria;Gil, Luis
通讯作者：
Gil, Luis