Expression profiling of exosomal miRNAs derived from human esophageal cancer cells by Solexa high-throughput sequencing.
Expression profiling of exosomal miRNAs derived from human esophageal cancer cells by Solexa high-throughput sequencing.
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通过 Solexa 高通量测序对人食管癌细胞来源的外泌体 miRNA 进行表达谱分析
DOI：
10.3390/ijms150915530
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发表时间：
2014-09-02
影响因子：
5.6
通讯作者：
Pu Y
中科院分区：
文献类型：
--
作者：
Liao J;Liu R;Yin L;Pu Y
细胞遗传物质，如微小RNA（miRNA）、mRNA和蛋白质，被包装在外泌体内，外泌体是内吞来源的小膜囊泡，其被释放到细胞外环境中。这些细胞遗传物质可以被递送到受体细胞中，在那里它们发挥各自的生物学效应。然而，癌细胞分泌的外泌体的miRNA谱和生物学功能仍然未知。本研究通过Solexa高通量测序技术，探讨人食管癌细胞来源exosomes的miRNA表达谱和分布特征。结果显示，细胞中有56，421个（2.94%）独特序列，外泌体中有7727个（0.63%）独特序列与已知的miRNA匹配。在细胞和外泌体中分别鉴定了总共342种和48种已知的miRNA。此外，在细胞和外泌体中分别预测了64和32种新的miRNA。在人食管癌细胞和外来体之间发现miRNA表达谱的显著差异。这些发现为研究人食管癌细胞来源的exosomes中miRNAs的特征以及miRNAs在食管癌中exosomes介导的细胞间通讯中的特定作用提供了新的见解。
Cellular genetic materials, such as microRNAs (miRNAs), mRNAs and proteins, are packaged inside exosomes, small membrane vesicles of endocytic origin that are released into the extracellular environment. These cellular genetic materials can be delivered into recipient cells, where they exert their respective biological effects. However, the miRNA profiles and biological functions of exosomes secreted by cancer cells remain unknown. The present study explored the miRNA expression profile and distribution characteristics of exosomes derived from human esophageal cancer cells through Solexa high-throughput sequencing. Results showed that 56,421 (2.94%) unique sequences in cells and 7727 (0.63%) in exosomes matched known miRNAs. A total of 342 and 48 known miRNAs were identified in cells and exosomes, respectively. Moreover, 64 and 32 novel miRNAs were predicted in cells and exosomes, respectively. Significant differences in miRNA expression profiles were found between human esophageal cancer cells and exosomes. These findings provided new insights into the characteristics of miRNAs in exosomes derived from human esophageal cancer cells and the specific roles of miRNAs in intercellular communication mediated by exosomes in esophageal cancer.
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作者：
Chiba M;Kimura M;Asari S
通讯作者：
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DOI：
10.1007/978-1-62703-453-1_2
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2013-01-01
期刊：
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10.7314/apjcp.2012.13.kksuppl.65
发表时间：
2012-01-01
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