Alarin stimulates food intake in male rats and LH secretion in castrated male rats.
Alarin stimulates food intake in male rats and LH secretion in castrated male rats.
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DOI：
10.1016/j.npep.2010.04.001
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发表时间：
2010-08
期刊：
影响因子：
2.9
通讯作者：
Fraley, Gregory S.
中科院分区：
文献类型：
--
作者：
Van Der Kolk, Nicole;Madison, Farrah N.;Mohr, Margaret;Eberhard, Nicole;Kofler, Barbara;Fraley, Gregory S.
丙氨酸是一种新发现的神经肽家族成员，包括丙氨酸样肽（GALP）和丙氨酸。在神经母细胞瘤细胞中发现Alarin是GALP基因的替代转录本，随后在啮齿动物的大脑中检测到Alarin mRNA。GALP和丙氨酸是摄食和繁殖行为的重要中枢调节因子。我们假设，作为甘丙肽家族的一员，甘丙肽对饲养和繁殖也有重要影响。为了验证这一假设，我们对雄性大鼠进行了脑室内注射，并测量了其对食物摄入、能量稳态、性行为和黄体生成素（LH）分泌的影响。结果显示，静脉注射1.0 nmol缬氨酸显著提高了大鼠的摄食量（p < 0.01）和体重（p < 0.05）。Alarin对雄性大鼠的性行为没有影响；然而，alarin显著（p < 0.01）提高了去势雄性大鼠的LH水平。在中脑蓝斑座和蓝斑亚座内检测到Alarin免疫反应细胞体，蓝斑亚座是脑干核，参与协调许多生理活动，包括食物摄取和生殖。最后，alarin刺激下丘脑核，如室旁核和孤束核的Fos诱导。我们的研究表明，像甘丙氨酸家族的其他成员一样，丙氨酸是食物摄入和体重的神经介质。
Alarin is a newly identified member of the galanin family of neuropeptides that includes galanin-like peptide (GALP) and galanin. Alarin was discovered as an alternate transcript of the GALP gene in neuroblastoma cells, and subsequently alarin mRNA was detected in the brain of rodents. GALP and galanin are important central regulators of both feeding and reproductive behavior. We hypothesized, that, as a member of the galanin family of peptides, alarin would also have central effects on feeding and reproduction. To test this hypothesis, we treated male rats with alarin intracerebroventricularly (i.c.v.) and measured its effects on food intake and energy homeostasis as well as sexual behavior and luteinizing hormone (LH) secretion. We observed that i.c.v. injection of 1.0 nmol alarin significantly increased food intake (p < 0.01) and body weight (p < 0.05). Alarin did not affect sexual behavior in male rats; however, alarin did significantly (p < 0.01) increase LH levels in castrated, but not intact, male rats. Alarin immunoreactive cell bodies were detected within the locus coeruleus and locus subcoeruleus of the midbrain, which is a brainstem nucleus involved in coordinating many physiological activities, including food intake and reproduction. Lastly, alarin stimulated Fos induction in hypothalamic nuclei, such as the paraventricular nucleus and the nucleus of the tractus solitarious. Our studies demonstrate that alarin, like other members of the galanin family, is a neuromediator of food intake and body weight.
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