Portable microsystem integrates multifunctional dielectrophoresis manipulations and a surface stress biosensor to detect red blood cells for hemolytic anemia.
Portable microsystem integrates multifunctional dielectrophoresis manipulations and a surface stress biosensor to detect red blood cells for hemolytic anemia.
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便携式微系统集成了多功能介电泳操作和表面应力生物传感器，用于检测溶血性贫血的红细胞
DOI：
10.1038/srep33626
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发表时间：
2016-09-20
影响因子：
4.6
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Sang S;Feng Q;Jian A;Li H;Ji J;Duan Q;Zhang W;Wang T
溶血性贫血强度已被认为是镰状细胞病某些临床并发症增长的重要因素。目前尚无快速有效的诊断方法。作为生物颗粒检测的强大平台，与微流体集成的生物传感器为新一代便携式床旁系统提供了巨大的潜力。在本文中，我们描述了一种新型的便携式微系统，包括一个多功能的介电电泳操作（MDM）设备和表面应力生物传感器分离和检测红细胞（RBC）的诊断溶血性贫血。为MDM器件的叉指电极阵列和表面应力生物传感器测试平台供电的外围电路被集成到便携式信号系统中。MDM包括准备区、聚焦区和分选区。模拟和实验结果显示了红细胞在受到正DEP力时的轨迹，允许成功分选活/死红细胞。然后将分离的RBC运输到生物传感器，并测量由表面应力的变化引起的电容值。溶血性贫血的诊断可以通过检测红细胞来实现，便携式微系统为溶血性贫血患者提供评估。
Hemolytic anemia intensity has been suggested as a vital factor for the growth of certain clinical complications of sickle cell disease. However, there is no effective and rapid diagnostic method. As a powerful platform for bio-particles testing, biosensors integrated with microfluidics offer great potential for a new generation of portable point of care systems. In this paper, we describe a novel portable microsystem consisting of a multifunctional dielectrophoresis manipulations (MDM) device and a surface stress biosensor to separate and detect red blood cells (RBCs) for diagnosis of hemolytic anemia. The peripheral circuit to power the interdigitated electrode array of the MDM device and the surface stress biosensor test platform were integrated into a portable signal system. The MDM includes a preparing region, a focusing region, and a sorting region. Simulation and experimental results show the RBCs trajectories when they are subjected to the positive DEP force, allowing the successful sorting of living/dead RBCs. Separated RBCs are then transported to the biosensor and the capacitance values resulting from the variation of surface stress were measured. The diagnosis of hemolytic anemia can be realized by detecting RBCs and the portable microsystem provides the assessment to the hemolytic anemia patient.
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