Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root.
Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root.
复制标题
DOI：
10.1126/science.adf4721
复制
发表时间：
2023-03-31
期刊：
影响因子：
56.9
通讯作者：
Benfey, Philip N.
中科院分区：
文献类型：
--
作者：
Nolan, Trevor M.;Vukasinovic, Nemanja;Hsu, Che-Wei;Zhang, Jingyuan;Vanhoutte, Isabelle;Shahan, Rachel;Taylor, Isaiah W.;Greenstreet, Laura;Heitz, Matthieu;Afanassiev, Anton;Wang, Ping;Szekely, Pablo;Brosnan, Aiden;Yin, Yanhai;Schiebinger, Geoffrey;Ohler, Uwe;Russinova, Eugenia;Benfey, Philip N.
油菜素内酯是植物类固醇激素，它通过在时空上变化的基因调控网络来调节多种过程，如细胞分裂和细胞伸长。通过使用时间序列单细胞RNA测序来分析拟南芥根不同细胞类型和发育阶段特异的油菜素内酯响应基因表达，我们确定伸长的皮层是油菜素内酯触发从增殖到伸长转变的部位，这与细胞壁相关基因表达增加有关。我们的分析揭示了拟南芥同源框7（HAT7）和类GT - 2 1（GTL1）是调节皮层细胞伸长的油菜素内酯响应转录因子。这些结果确定皮层是油菜素内酯介导生长的部位，并揭示了一个调节从增殖到伸长转变的油菜素内酯信号网络，阐明了激素时空响应的各个方面。
单细胞RNA测序确定皮层是拟南芥根中油菜素内酯依赖生长的部位。
Brassinosteroids are plant steroid hormones that regulate diverse processes such as cell division and cell elongation through gene regulatory networks that vary in space and time. By using time-series single-cell RNA-sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall-related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, illuminating aspects of spatiotemporal hormone response. Single-cell RNA-sequencing identifies the cortex as a site of brassinosteroid-dependent growth in Arabidopsis roots.
登录
查看更多内容
影响因子：
9.5
作者：
Moreno-Risueno MA;Busch W;Benfey PN
通讯作者：
Benfey PN
影响因子：
7.7
作者：
Barbier de Reuille P;Routier-Kierzkowska AL;Kierzkowski D;Bassel GW;Schüpbach T;Tauriello G;Bajpai N;Strauss S;Weber A;Kiss A;Burian A;Hofhuis H;Sapala A;Lipowczan M;Heimlicher MB;Robinson S;Bayer EM;Basler K;Koumoutsakos P;Roeder AH;Aegerter-Wilmsen T;Nakayama N;Tsiantis M;Hay A;Kwiatkowska D;Xenarios I;Kuhlemeier C;Smith RS
通讯作者：
Smith RS
影响因子：
4.4
作者：
Seyed Rahmani R;Shi T;Zhang D;Gou X;Yi J;Miclotte G;Marchal K;Li J
通讯作者：
Li J
影响因子：
7.4
作者：
Asami, T;Min, YK;Yoshida, S
通讯作者：
Yoshida, S
影响因子：
9.9
作者：
通讯作者：
--