Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies.
Supplemental Dietary Selenohomolanthionine Improve Antioxidant Activity and Immune Function in Weaned Beagle Puppies.
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补充膳食硒高羊毛硫氨酸可改善断奶比格犬幼犬的抗氧化活性和免疫功能
DOI：
10.3389/fvets.2021.728358
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发表时间：
2021
影响因子：
3.2
通讯作者：
Song H
中科院分区：
文献类型：
--
作者：
Shao C;Zheng M;Yu Z;Jiang S;Zhou B;Song Q;Ma T;Zhou Y;Dong W;Li D;Gu Y;Wang X;Song H
本研究旨在探讨硒高羊毛硫氨酸（SeHLan）对犬细小病毒（CPV）免疫幼犬抗氧化状态和免疫应答的影响。在这项研究中，30只断奶幼犬被随机分为六组：对照组（-Se/-Vacc），免疫组（-Se/+Vacc），补充亚硒酸钠组（SS/+Vacc，0.35 mg/kg DM），低剂量SeHLan组（SeHLan-L/+Vacc，0.35 mg/kg DM）、中剂量SeHLan组（SeHLan-M/+Vacc，1.0 mg/kg DM）和高剂量SeHLan组（SeHLan-H/+Vacc，2.0 mg/kg DM）。幼犬喂养42天，并在第0天和第21天接种Vanguard Plus 5疫苗。分别于免疫后7、14、21、28、35、42 d采血，测定抗氧化指标、淋巴细胞增殖指数、血清细胞因子（IL-2、IL-4）浓度、犬中性粒细胞（PMN）吞噬功能和犬细小病毒抗体滴度。结果表明，硒化羟蓝可显著提高血清硒浓度和谷胱甘肽过氧化物酶（GSH-Px）活性（P < 0.05），且呈剂量依赖关系。同时，还能提高血清超氧化物歧化酶（SOD）活性，降低血清丙二醛（MDA）含量，以SeHLan-M/+Vacc（1.0mg/kg DM）组效果最好（P < 0.01）。硒化壳聚糖能显著提高免疫犬血清中淋巴细胞增殖、IL-2和IL-4水平，增强PMN的吞噬功能（P < 0.05）。SeHLan可缩短CPV抗体产生时间，提高抗体滴度（P < 0.05）。值得注意的是，SeHLan的有益效果上级SS。总之，日粮中补充硒HLan可提高断奶后幼犬的抗氧化活性，增加CPV抗体滴度，增强免疫功能。适宜剂量的硒化蓝（1~2 mg/kg DM）对幼犬有一定的营养价值。
The purpose of this study was to investigate the effects of dietary Selenohomolanthionine (SeHLan) on antioxidant status and immune response in canine parvovirus (CPV) vaccinated puppies. In this study, 30 weaned puppies were randomly divided into six groups: control group (–Se/–Vacc), immunization group (–Se/+Vacc), supplementation of sodium selenite group (SS/+Vacc, 0.35 mg/kg DM), low-dose SeHLan group (SeHLan-L/+Vacc, 0.35 mg/kg DM), mid-dose SeHLan group (SeHLan-M/+Vacc, 1.0 mg/kg DM), and high-dose SeHLan group (SeHLan-H/+Vacc, 2.0 mg/kg DM). The puppies were fed for 42 days and vaccinated with Vanguard Plus 5 on day 0 and day 21. Blood samples were collected on 7, 14, 21, 28, 35, 42 days post-immunization (PI) for determination of antioxidant indicators, lymphocyte proliferation index, serum cytokine concentration (IL-2, IL-4), canine polymorphonuclear neutrophils (PMN) phagocytic function, and the level of CPV antibody titers. The results showed that SeHLan supplementation raised the serum Se concentration and glutathione peroxidase (GSH-Px) activity in a dose-dependent manner (P < 0.05). It also increased the activity of serum superoxide dismutase (SOD) and decreased serum malondialdehyde (MDA) content, especially in SeHLan-M/+Vacc group (1.0 mg/kg DM) (P < 0.01). SeHLan supplementation significantly increased lymphocyte proliferation, IL-2, and IL-4 levels in canine serum, and enhanced phagocytosis of PMN in vaccinated puppies (P < 0.05). Moreover, SeHLan supplementation shortened the CPV antibody production time and increased the CPV antibody titers (P < 0.05). Of note, the beneficial effects of SeHLan were superior to those of SS. In conclusion, dietary SeHLan supplementation improved antioxidant activity, increased CPV antibody titers, and enhanced immune function in puppies after weaning. An appropriate dosage of SeHLan (1~2 mg/kg DM) may confer nutritional benefits in puppies.
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影响因子：
5.2
作者：
Hoffmann, Peter R.;Berry, Marla J.
通讯作者：
Berry, Marla J.
DOI：
10.1016/s0147-9571(99)00066-1
发表时间：
2000-03-01
影响因子：
2
作者：
Giadinis, N;Koptopoulos, G;Papasteriades, A
通讯作者：
Papasteriades, A
影响因子：
3.2
作者：
Liu, Kangjun;Ding, Tao;Li, Jianji
通讯作者：
Li, Jianji
影响因子：
2.2
作者：
Kono, Mari;Saigo, Katsuyasu;Kawano, Seiji
通讯作者：
Kawano, Seiji
影响因子：
5.9
作者：
Donadio JL;Guerra-Shinohara EM;Rogero MM;Cozzolino SM
通讯作者：
Cozzolino SM