Simultaneous editing of three homoeologues of TaCIPK14 confers broad-spectrum resistance to stripe rust in wheat.
Simultaneous editing of three homoeologues of TaCIPK14 confers broad-spectrum resistance to stripe rust in wheat.
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DOI：
10.1111/pbi.13956
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发表时间：
2023-03
影响因子：
13.8
通讯作者：
Guo, Jun
中科院分区：
文献类型：
--
作者：
He, Fuxin;Wang, Ce;Sun, Huilin;Tian, Shuxin;Zhao, Guosen;Liu, Cong;Wan, Cuiping;Guo, Jia;Huang, Xueling;Zhan, Gangming;Yu, Xiumei;Kang, Zhensheng;Guo, Jun
关键词：
小麦条锈病是由小麦条锈菌（Pucciniastriiformis f.）小麦锈病是小麦生产上最具破坏性的病害之一，在世界范围内造成了严重的损失。培育抗病品种是控制病害最经济有效的措施。通过CRISPR/Cas9介导的基因编辑技术改变促进病原体相容性的易感基因已成为培育抗病小麦品种的新策略。钙调磷酸酶B样蛋白（CBL）相互作用蛋白激酶（CIPKs）已被证明参与植物病原体相互作用期间的防御反应。然而，小麦CIPK是否作为易感因子仍不清楚。在此，我们从小麦中分离到一个CIPK同源基因TaCIPK 14。TaCIPK 14的敲低显著增加了小麦对Pst的抗性，而TaCIPK 14的过表达通过降低防御反应的不同方面，包括ROS的积累和致病相关基因的表达，导致小麦对Pst的易感性增强。我们通过CRISPR/Cas9技术同时修饰小麦TaCIPK 14的三个同源物，获得了小麦Tacipk 14突变体植物。Tacipk 14突变株系对Pst表达了非种族特异性（RNS）广谱抗性（BSR）。此外，在温室和田间条件下，Tacipk 14突变体植株和Fielder对照植株的农艺产量性状没有发现显著差异。这些结果表明，TaCIPK 14是小麦对Pst反应的重要易感因子，敲除TaCIPK 14代表了产生具有对Pst的BSR的新抗病小麦品种的强有力策略。
Wheat stripe rust caused by the fungus Puccinia striiformis f. sp. tritici (Pst) is one of the most destructive wheat diseases resulting in significant losses to wheat production worldwide. The development of disease‐resistant varieties is the most economical and effective measure to control diseases. Altering the susceptibility genes that promote pathogen compatibility via CRISPR/Cas9‐mediated gene editing technology has become a new strategy for developing disease‐resistant wheat varieties. Calcineurin B‐like protein (CBL)‐interacting protein kinases (CIPKs) has been demonstrated to be involved in defence responses during plant‐pathogen interactions. However, whether wheat CIPK functions as susceptibility factor is still unclear. Here, we isolated a CIPK homoeologue gene TaCIPK14 from wheat. Knockdown of TaCIPK14 significantly increased wheat resistance to Pst, whereas overexpression of TaCIPK14 resulted in enhanced wheat susceptibility to Pst by decreasing different aspects of the defence response, including accumulation of ROS and expression of pathogenesis‐relative genes. We generated wheat Tacipk14 mutant plants by simultaneous modification of the three homoeologues of wheat TaCIPK14 via CRISPR/Cas9 technology. The Tacipk14 mutant lines expressed race‐nonspecific (RNS) broad‐spectrum resistance (BSR) to Pst. Moreover, no significant difference was found in agronomic yield traits between Tacipk14 mutant plants and Fielder control plants under greenhouse and field conditions. These results demonstrate that TaCIPK14 acts as an important susceptibility factor in wheat response to Pst, and knockout of TaCIPK14 represents a powerful strategy for generating new disease‐resistant wheat varieties with BSR to Pst.
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