Hydrophobic constituents and their potential anticancer activities from Devil's Club (Oplopanax horridus Miq.).
Hydrophobic constituents and their potential anticancer activities from Devil's Club (Oplopanax horridus Miq.).
复制标题
疏水成分及其潜在的抗癌活动来自Devil's Club（Oplopanax Horridus Miq。）。
DOI：
10.1016/j.jep.2010.08.026
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发表时间：
2010-10-28
影响因子：
5.4
通讯作者：
Yuan, Chun-Su
中科院分区：
文献类型：
--
作者：
Sun, Shi;Du, Guang-Jian;Qi, Lian-Wen;Williams, Stainley;Wang, Chong-Zhi;Yuan, Chun-Su
关键词：
刺人参Oplopanax horridus（Sm.）MIQ.是一种广泛使用的民间医药，在太平洋西北部，如阿拉斯加和不列颠哥伦比亚省，用于治疗各种疾病，包括关节炎，感冒，发烧，感染，糖尿病和癌症。目的研究魔鬼俱乐部O中的疏水性成分及其潜在的抗癌活性。可怕的东西。其根皮为O. horridus（Sm.）通过色谱技术分离Miq。分离的化合物的结构进行了鉴定，通过光谱方法和与已发表的数据进行比较。检测了分离的疏水组分在人乳腺癌MCF-7细胞、人结肠癌SW-480和HCT-116细胞中的抗增殖作用。采用细胞周期和细胞凋亡分析探讨了其抗增殖作用的可能机制。分离得到6个化合物，分别鉴定为9，17-十八碳二烯-12，14-二炔-1，11，16-三醇，1-乙酸酯（1），oplopandiol acetate（2），falcarindiol（3），oplopandiol（4），trans-nerolidol（5）和t-cadinol（6）。这些化合物对人乳腺癌和结肠癌细胞具有潜在的抗癌活性，其中化合物3的活性最强。流式细胞仪进一步检测细胞周期和凋亡，结果显示聚乙炔1-4可诱导HCT-116细胞阻滞于G2/M期，并通过诱导细胞凋亡抑制细胞增殖。这些结果为O. horridus以及一些用于治疗癌症的分离化合物。
Devil’s Club Oplopanax horridus (Sm.) Miq. is a widely used folk medicine in the Pacific Northwest such as Alaska and British Columbia for treating a variety of ailments including arthritis, cold, fever, infections, diabetes and cancer. To investigate hydrophobic constituents and their potential anticancer activity from Devil’s Club O. horridus. The root bark of O. horridus (Sm.) Miq was isolated by chromatographic techniques. Structures of isolated compounds were identified by spectroscopic methods and comparison with published data. The anti-proliferation of isolated hydrophobic constituents in human breast cancer MCF-7 cells, human colon cancer SW-480 and HCT-116 cells were tested. The potential mechanism of anti-proliferation was also investigated using cell cycle and apoptosis assays. Six compounds were isolated and structurally identified as 9,17-octadecadiene-12,14-diyne-1,11,16-triol, 1-acetate (1), oplopandiol acetate (2), falcarindiol (3), oplopandiol (4), trans-nerolidol (5) and t-cadinol (6). These compounds showed potential anticancer activities on human breast cancer and colon cancer cells, of which compound 3 possesses the strongest activity. Further cell cycle and apoptosis test by flow cytometry showed the polyacetylenes 1–4 induced HCT-116 cell arresting in G2/M phase and inhibited proliferation by the induction of apoptosis at both earlier and later stage. These results provide promising baseline information for the potential use of O. horridus as well as some of the isolated compounds in the treatment of cancer.
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