Cordyceps sinensis: In vitro anti-fibrotic bioactivity of natural and cultured preparations
Cordyceps sinensis: In vitro anti-fibrotic bioactivity of natural and cultured preparations
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冬虫夏草：天然和培养制剂的体外抗纤维化生物活性
DOI：
10.1016/j.foodhyd.2013.06.023
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发表时间：
2014-03
影响因子：
10.7
通讯作者：
Aled O. Phillips
中科院分区：
文献类型：
--
作者：
Soma Meran;Yapeng Fang;John Martin;Adam Midgley;Ming-Ming Pan;Bi-Cheng Liu;Steve W. Cui;Glyn O. Phillips;Aled O. Phillips
传统中草药冬虫夏草（Cordyceps sinensis，CS）对肾脏疾病有一定的治疗作用。我们以前已经证明了天然存在的CS的生物活性。为了满足日益增长的需求，CS衍生物已经通过无菌菌丝体培养来生产。本研究的目的是比较天然虫草素和冬虫夏草菌丝体培养产物“金水宝胶囊”（Paecilomyces sinensis）和“虫草胶囊”（Hirsutella sinensis）的生物活性。此外，我们使用两种不同的细胞系统检测了这些产品在肾脏以外的潜在抗纤维化潜力。所有三种制剂都拮抗了肾上皮细胞中促纤维化细胞因子TGF-β1的活性。在原水溶性组分以及各制剂的亲水性和疏水性提取物中观察到活性，表明生物活性不限于原材料的一种组分。除了在肾细胞系中的作用外，还观察到TGF-β1对肺成纤维细胞表型活化的拮抗作用相似，表明这些观察结果适用于肾脏以外的实体器官纤维化。从机制上讲，数据证明了TGF-β1对Smad信号传导的激活的拮抗作用，特别是对透明质酸粘附蛋白TSG-6的诱导的拮抗作用，我们以前已经证明了透明质酸的形成，总之，数据支持天然存在的和来自培养的虫草菌丝体的产品具有相似的生物活性的说法。活性可能与实体器官纤维化和相关器官功能障碍特别相关。
It has been suggested that the Traditional Chinese herbal preparationCordyceps sinensis(CS) may have a beneficial effect in renal disease. We have previously demonstrated bio-activity of naturally occurring CS. To satisfy increasing demand, CS derivatives have been produced by aseptic mycelia cultivation. The aim of this work was to compare and contrast the bio-activity of natural CS and two specific products derived from culturedCordyceps mycelia, “Jinshuibao Capsule” (Paecilomyces sinensis) and “Corbrin Capsule” (Hirsutella sinensis). In addition using two different cell systems we examined the potential anti-fibrotic potential of these products beyond the kidney.All three preparations antagonised the activity of the pro-fibrotic cytokine TGF-β1 in renal epithelial cells. Activity was seen in the raw water-soluble components, and the hydrophilic and hydrophobic extracts from each preparation suggesting that the bio-activity is not confined to one component of the raw material. In addition to the effects in the renal cell line, similar effects of antagonism of phenotypic activation of pulmonary fibroblasts by TGF-β1 was also seen suggesting an applicability of these observations to solid organ fibrosis beyond the kidney. Mechanistically the data demonstrate antagonism of TGF-β1 activation of Smad signalling and specifically of induction of the hyaladherin TSG-6, which we have previously demonstrated to orchestrate the formation of a hyaluronan, peri-cellular coats driving phenotypic cellular activation.In summary the data support the claim that both naturally occurring and products derived from culturedCordyceps myceliahave similar bio-activity which may have particular relevance for solid organ fibrosis and associated organ dysfunction.
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影响因子：
--
作者：
X. Zhao;L. Li
通讯作者：
X. Zhao;L. Li
DOI：
--
发表时间：
2007-12
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
Shao-jie Wang;Wen Bai;Chun-ling Wang;Zhong Dai
通讯作者：
Shao-jie Wang;Wen Bai;Chun-ling Wang;Zhong Dai
影响因子：
2.4
作者：
Cui, J. D.;Zhang, B. Z.
通讯作者：
Zhang, B. Z.
培养冬虫夏草中分离的酸性多糖组分对体外巨噬细胞的影响
影响因子：
4.3
作者：
Chen, Weixia;Zhang, Weiyun;Wang, Kongcheng
通讯作者：
Wang, Kongcheng
影响因子：
19.6
作者：
Ng, YY;Huang, TP;Lan, HY
通讯作者：
Lan, HY