Broad Distribution and Phylogeny of Anaerobic Endophytes of Cluster XIVa Clostridia in Plant Species Including Crops
Broad Distribution and Phylogeny of Anaerobic Endophytes of Cluster XIVa Clostridia in Plant Species Including Crops
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XIVa 梭状芽胞杆菌厌氧内生菌在包括农作物在内的植物物种中的广泛分布和系统发育
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Inamisawa
中科院分区：
文献类型：
--
作者：
Sami;Aito;Akoto;awahara;Eishi;Keda;Asato;Shimine;Hoichiro;Kao;Iwamu;Inamisawa
通过对梭菌16S rRNA基因特异性的末端限制性片段长度多态性（TRFLP）分析，调查了作为专性厌氧菌存在于各种植物上的内生梭菌。在甘蔗、栽培稻、玉米、烟草、大豆、狗牙根、高羊茅和3种红树林植物中检测到内生梭菌。系统发育，集群XIVa梭菌比集群I梭菌在地上部分检测更频繁。从表面灭菌的甘蔗茎中分离梭菌验证了TRFLP结果。植物来源的梭菌占据了两个独特的系统发育位置（组I和II）内集群XIVa。大多数簇XIVa梭菌来自其他来源（例如，人、动物和昆虫肠）位于这些组之外。因此，XIVa簇梭菌的两个独特类群广泛分布在植物中，包括农作物。在田间种植的大豆，TRFLP分析显示梭菌只在一个非发酵突变体。核糖体基因间间隔区分析（RISA）表明，大豆地上部细菌群落结构部分取决于大豆根瘤菌的基因型。
Endophytic clostridia present on various plants as obligate anaerobes were surveyed by terminal restriction fragment length polymorphism (TRFLP) analysis specific to the clostridial 16S rRNA gene. Endophytic clostridia were detected in 10 plant types: sugarcane, cultivated rice, corn, tobacco, soybean, bermuda grass, tall fescue, and three mangrove species. Phylogenetically, cluster XIVa clostridia were detected more frequently than cluster I clostridia in aerial parts. Isolation of clostridia from surface-sterilized sugarcane stem validated the TRFLP results. Plant-derived clostridia occupied two unique phylogenetic positions (groups I and II) within cluster XIVa. Most of cluster XIVa clostridia from other sources (e.g., human, animal, and insect intestines) were located outside these groups. Thus two unique groups of cluster XIVa clostridia are widely distributed in plants, including crops. In field-grown soybeans, TRFLP analysis revealed clostridia only in a non-nodulating mutant. Ribosomal intergenic spacer analysis (RISA) showed that the bacterial community in soybean shoot depended partly on the soybean nodulation genotype.
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影响因子：
2.2
作者：
M. Zakria;J. Njoloma;Y. Saeki;S. Akao
通讯作者：
M. Zakria;J. Njoloma;Y. Saeki;S. Akao
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE
影响因子：
2.9
作者：
Taksawan Thongaram;Yuichi Hongoh;S. Kosono;M. Ohkuma;Savitr Trakulnaleamsai;N. Noparatnaraporn;T. Kudo
通讯作者：
Taksawan Thongaram;Yuichi Hongoh;S. Kosono;M. Ohkuma;Savitr Trakulnaleamsai;N. Noparatnaraporn;T. Kudo
影响因子：
2.2
作者：
Mano, Hironobu;Tanaka, Fumiko;Morisaki, Hisao
通讯作者：
Morisaki, Hisao
影响因子：
4.4
作者：
Miyamoto, T;Kawahara, M;Minamisawa, K
通讯作者：
Minamisawa, K