Viunalikeviruses are environmentally common agents of horizontal gene transfer in pathogens and biocontrol bacteria.
Viunalikeviruses are environmentally common agents of horizontal gene transfer in pathogens and biocontrol bacteria.
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DOI：
10.1038/ismej.2014.150
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发表时间：
2014-10
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影响因子：
--
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文献类型：
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Bacteriophages have been used as natural biocontrol and therapeutic agents, but also as biotechnological tools for bacterial engineering. We showed recently that the transducing bacteriophage ϕMAM1 is a ViI-like phage and a member of the new genus, ‘Viunalikevirus'. Here, we show that four additional ViI-like phages and three new environmentally isolated viunalikeviruses, all infecting plant and human pathogens, are very efficient generalised transducers capable of transducing chromosomal markers at frequencies of up to 10−4 transductants per plaque-forming unit. We also demonstrate the interstrain transduction of plasmids and chromosomal markers, including genes involved in anabolism, genes for virulence and genes encoding secondary metabolites involved in biocontrol. We propose that all viunalikeviruses are likely to perform efficient horizontal gene transfer. Viunalikeviruses therefore represent useful agents for functional genomics and bacterial engineering, and for chemical and synthetic biology studies, but could be viewed as inappropriate choices for phage therapy.
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影响因子：
4.8
作者：
Hooton SP;Timms AR;Rowsell J;Wilson R;Connerton IF
通讯作者：
Connerton IF
DOI：
10.1073/pnas.0808832106
发表时间：
2009-01-20
影响因子：
11.1
作者：
Fineran, Peter C.;Blower, Tim R.;Salmond, George P. C.
通讯作者：
Salmond, George P. C.
影响因子：
2.7
作者：
Adriaenssens, Evelien M.;Ackermann, Hans-Wolfgang;Anany, Hany;Blasdel, Bob;Connerton, Ian F.;Goulding, David;Griffiths, Mansel W.;Hooton, Steven P.;Kutter, Elizabeth M.;Kropinski, Andrew M.;Lee, Ju-Hoon;Maes, Martine;Pickard, Derek;Ryu, Sangryeol;Sepehrizadeh, Zargham;Shahrbabak, S. Sabouri;Toribio, Ana L.;Lavigne, Rob
通讯作者：
Lavigne, Rob
影响因子：
2.7
作者：
ACKERMANN, HW;BROCHU, G;KONJIN, HPE
通讯作者：
KONJIN, HPE
影响因子：
14.9
作者：
Blower TR;Short FL;Rao F;Mizuguchi K;Pei XY;Fineran PC;Luisi BF;Salmond GP
通讯作者：
Salmond GP