Cell-cell interactions during the formation of primordial follicles in humans.
Cell-cell interactions during the formation of primordial follicles in humans.
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人类原始卵泡形成过程中的细胞 - 细胞相互作用。
DOI：
10.26508/lsa.202301926
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发表时间：
2023-11
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
这项研究揭示了卵巢索中多核合胞体和细胞间桥的存在，并强调了细胞间相互作用在人类原始卵泡形成过程中的作用。配子发生是一个复杂且性别特异性的多步骤过程，在此过程中性腺体细胞生态位发挥着重要的调节作用。人类雌性配子发生过程中最关键的步骤之一是原始卵泡的形成，原始卵泡是卵巢的功能单位，构成整个生殖生命中出生时可用的卵泡库。然而，人类胎儿生殖细胞（hFGC）和性腺体细胞在原始卵泡形成过程中的关系在很大程度上仍未被探索。我们发现 hFGC 可以形成多核合胞体，其中一些通过互连细胞间桥连接，并且并非 hFGC 合胞体中的所有核在减数分裂阶段都是同步的。随着 hFGC 发育的进展，前颗粒细胞形成突起，似乎逐渐收缩单个 hFGC，可能有助于将它们与多核合胞体分离。我们的研究结果强调了人类原始卵泡形成过程中 hFGC 和（前）颗粒细胞之间的细胞间相互作用和分子动力学。了解原始卵泡池如何形成对于理解人类不孕症非常重要。
This study revealed the presence of multinucleated syncytia and intercellular bridges in ovarian cords and highlighted the role of cell–cell interactions during primordial follicle formation in humans. Gametogenesis is a complex and sex-specific multistep process during which the gonadal somatic niche plays an essential regulatory role. One of the most crucial steps during human female gametogenesis is the formation of primordial follicles, the functional unit of the ovary that constitutes the pool of follicles available at birth during the entire reproductive life. However, the relation between human fetal germ cells (hFGCs) and gonadal somatic cells during the formation of the primordial follicles remains largely unexplored. We have discovered that hFGCs can form multinucleated syncytia, some connected via interconnecting intercellular bridges, and that not all nuclei in hFGC–syncytia were synchronous regarding meiotic stage. As hFGCs progressed in development, pre-granulosa cells formed protrusions that seemed to progressively constrict individual hFGCs, perhaps contributing to separate them from the multinucleated syncytia. Our findings highlighted the cell–cell interaction and molecular dynamics between hFGCs and (pre)granulosa cells during the formation of primordial follicles in humans. Knowledge on how the pool of primordial follicle is formed is important to understand human infertility.
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