Characterization of the bacterial community associated with larvae and adults of Anoplophora chinensis collected in Italy by culture and culture-independent methods.
Characterization of the bacterial community associated with larvae and adults of Anoplophora chinensis collected in Italy by culture and culture-independent methods.
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DOI：
10.1155/2013/420287
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发表时间：
2013
影响因子：
--
通讯作者：
Daffonchio D
中科院分区：
文献类型：
--
作者：
Rizzi A;Crotti E;Borruso L;Jucker C;Lupi D;Colombo M;Daffonchio D
中国星天牛（Anoplophora chinensis Forster）是一种蛀木甲虫，原产于中国，近年来已蔓延到北美和欧洲，对观赏树木和林木造成严重危害。与这些食木甲虫肠道微生物群落的兴趣是潜在的生物技术应用在木质纤维素降解和害虫防治措施的发展。本研究对中华绒螯蟹幼虫和成虫的肠道细菌群落进行了研究。采用培养法和非培养法对采自意大利北部不同寄主树木的中国小蠊进行了调查。幼虫和成人窝藏一个适度多样化的细菌群落，占主导地位的变形菌，放线菌，厚壁菌门。最具代表性的是γ-变形菌科肠杆菌科（Gibbsiella、Enterobacter、Raoultella和Klebsiella属）。这种细菌在昆虫肠道中的丰度可能是由于肠杆菌科的各种代谢能力，包括来源于木质纤维素降解的碳水化合物的发酵和通过固氮活性对氮摄入的贡献。此外，先前显示具有一些木质纤维素降解活性的细菌在肠道中以相对低的水平检测到。这些细菌在木质纤维素分解中可能与内源酶和真菌酶协同作用。放线菌共生体的检测可以解释为在植物次生代谢产物的解毒和/或对病原体的保护中的可能作用。
The wood-boring beetle Anoplophora chinensis Forster, native to China, has recently spread to North America and Europe causing serious damage to ornamental and forest trees. The gut microbial community associated with these xylophagous beetles is of interest for potential biotechnological applications in lignocellulose degradation and development of pest-control measures. In this study the gut bacterial community of larvae and adults of A. chinensis, collected from different host trees in North Italy, was investigated by both culture and culture-independent methods. Larvae and adults harboured a moderately diverse bacterial community, dominated by Proteobacteria, Actinobacteria, and Firmicutes. The gammaproteobacterial family Enterobacteriaceae (genera Gibbsiella, Enterobacter, Raoultella, and Klebsiella) was the best represented. The abundance of such bacteria in the insect gut is likely due to the various metabolic abilities of Enterobacteriaceae, including fermentation of carbohydrates derived from lignocellulose degradation and contribution to nitrogen intake by nitrogen-fixing activity. In addition, bacteria previously shown to have some lignocellulose-degrading activity were detected at a relatively low level in the gut. These bacteria possibly act synergistically with endogenous and fungal enzymes in lignocellulose breakdown. The detection of actinobacterial symbionts could be explained by a possible role in the detoxification of secondary plant metabolites and/or protection against pathogens.
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DOI：
10.1073/pnas.0610451104
发表时间：
2007-05-22
影响因子：
11.1
作者：
Favia, Guido;Ricci, Irene;Daffonchio, Daniele
通讯作者：
Daffonchio, Daniele
影响因子：
4.9
作者：
CAVALCANTE, VA;DOBEREINER, J
通讯作者：
DOBEREINER, J
影响因子：
3.4
作者：
Gruenwald, S.;Pilhofer, M.;Hoell, W.
通讯作者：
Hoell, W.
影响因子：
4.9
作者：
Behar, A;Yuval, B;Jurkevitch, E
通讯作者：
Jurkevitch, E
影响因子：
4.4
作者：
Gonella, Elena;Negri, Ilaria;Daffonchio, Daniele
通讯作者：
Daffonchio, Daniele