Ethyl pyruvate ameliorate inflammatory response of sinonasal mucosa by inhibiting HMGB1 in rats with acute rhinosinusitis.
Ethyl pyruvate ameliorate inflammatory response of sinonasal mucosa by inhibiting HMGB1 in rats with acute rhinosinusitis.
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丙酮酸乙酯通过抑制HMGB1改善急性鼻窦炎大鼠鼻窦黏膜炎症反应
DOI：
10.1038/s41598-021-85785-3
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发表时间：
2021-03-18
影响因子：
4.6
通讯作者：
Tan G
中科院分区：
文献类型：
--
作者：
Liang X;Shen Y;Zhang X;He G;Tan G
已知高迁移率族蛋白 1 (HMGB1) 参与许多炎症性疾病的发病机制。本研究的目的是建立急性鼻窦炎（ARS）动物模型，并确定丙酮酸乙酯（EP）是否通过抑制ARS动物中的HMGB1来减轻鼻窦粘膜的炎症反应。使用 36 只 Sprague Dawley (SD) 大鼠，如下： 6 只正常对照，无干预（第 1 组）； 30只大鼠通过鼻塞Merocel海绵建立ARS大鼠模型，模型大鼠不做任何处理（第2组），分别滴鼻无菌生理盐水（第3组）、10μl EP（第4组）和20μl EP（第5组），每天2次，连续5天。定期进行细菌培养，并使用基质辅助激光解吸/电离飞行时间质谱法鉴定主要菌株。采用免疫组化和RT-PCR检测鼻窦黏膜HMGB1的表达。通过 ELISA 测定血清 HMGB1、IL-6 和 TNF-α 水平。对已完成研究的 36 只大鼠中 29 只的数据进行了分析。与对照组相比，各组 ARS 大鼠鼻分泌物的菌落形成单位（CFU）显着较高（p<0.001）。与单独用盐水治疗的大鼠相比，用EP治疗的ARS大鼠的CFU仅略有下降，但鼻窦粘膜的炎症反应显着减弱，HMGB1表达降低（p<0.001）。与对照组相比，ARS 大鼠的 HMGB1、IL-6 和 TNF-α 血清水平显着升高，并且通过 EP 治疗而降低（p<0.001）。鼻海绵填塞导致大鼠鼻窦急性炎症反应，并增加HMGB1、IL-6和TNF-α的表达。 EP滴鼻液可通过抑制ARS大鼠HMGB1、IL-6、TNF-α的表达，减轻鼻窦黏膜炎症。
High mobility group box 1 (HMGB1) has been known to involve in the pathogenesis of many inflammatory diseases. The aim of this study was to establish animal model of acute rhinosinusitis (ARS), and determine whether ethyl pyruvate (EP) attenuate inflammatory response of sinonasal mucosa by inhibiting HMGB1 in ARS animals. Thirty-six Sprague Dawley (SD) rat were used as follows: six normal controls without intervention (group 1); thirty rats were used for establishment of ARS rats model by nasal insertion of Merocel sponge, and model rats without any treatments (group 2), treated with nasal drops of sterile saline (group 3), 10 μl EP (group 4), and 20 μl EP (group 5), twice a day for 5 days, respectively. Bacterial culture was done regularly and the main bacterial strains were identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry. HMGB1 expression in sinonasal mucosa was detected by immunohistochemistry and RT-PCR. Serum levels of HMGB1, IL-6, and TNF-α were determined by ELISA. Data from 29 of 36 rats that had completed research were analyzed. Bacterial colony formation unit (CFU) of nasal secretion was significantly higher in each group of ARS rats compared with controls (p < 0.001). ARS rats treated with EP had only slightly decreased CFU, but significantly attenuated inflammatory response of sinonasal mucosa and decreased HMGB1 expression compared to those treated with saline alone (p < 0.001). Serum levels of HMGB1, IL-6 and TNF-α were significantly higher in ARS rats compared to controls, and decreased by EP treatments (p < 0.001). Nasal sponge packing led to acute inflammatory response of nasal sinus in rats, and increased the expression of HMGB1, IL-6, and TNF-α. Nasal drops with EP could attenuate the inflammation of sinonasal mucosa through inhibiting the expression of HMGB1, IL-6 and TNF-α in ARS rats.
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DOI：
10.1186/s12950-016-0144-1
发表时间：
2016
期刊：
Journal of inflammation (London, England)
影响因子：
--
作者：
Yang R;Zhu S;Tonnessen TI
通讯作者：
Tonnessen TI
影响因子：
6.4
作者：
Rrapaj, Eltjona;Trisolini, Elena;Boldorini, Renzo L.
通讯作者：
Boldorini, Renzo L.
鼻孔炎的实际概念：临床表现，炎症途径，细胞因子谱，重塑和管理的综述。
影响因子：
5.5
作者：
Eloy P;Poirrier AL;De Dorlodot C;Van Zele T;Watelet JB;Bertrand B
通讯作者：
Bertrand B
影响因子：
2.8
作者：
Klemens, Christine;Rasp, Gerd;Kramer, Matthias F.
通讯作者：
Kramer, Matthias F.
影响因子：
6.1
作者：
Riechelmann, H;Deutschle, T;Bürner, H
通讯作者：
Bürner, H