Dramatic transcriptional changes in an intracellular parasite enable host switching between plant and insect.
Dramatic transcriptional changes in an intracellular parasite enable host switching between plant and insect.
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DOI：
10.1371/journal.pone.0023242
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Namba S
中科院分区：
文献类型：
--
作者：
Oshima K;Ishii Y;Kakizawa S;Sugawara K;Neriya Y;Himeno M;Minato N;Miura C;Shiraishi T;Yamaji Y;Namba S
植原体是一种细菌性植物病原体，对世界各地的农作物和植物的产量都有毁灭性的影响。它们是植物和昆虫的胞内寄生虫，通过昆虫在植物中传播。植原体如何适应两种不同的环境引起了人们的极大兴趣；然而，使植物和昆虫宿主之间的“宿主切换”成为可能的机制却鲜为人知。在这里，我们报告了植原体在植物和昆虫之间的“寄主转换”反应中戏剧性地改变其基因表达。我们详细描述了星状念珠菌Oy-M株在寄主植物和昆虫之间切换时发生的基因表达谱的戏剧性变化(大约33%的基因变化)。植原体可以以宿主特有的方式使用转运蛋白、分泌的蛋白质和代谢酶。由于植原体存在于宿主细胞内，植原体分泌的蛋白质被认为在植原体与宿主细胞之间的相互作用中起着至关重要的作用。我们的基因芯片分析表明，编码分泌蛋白PAM486的基因在植物寄主中表达高度上调，免疫组织化学分析也观察到这一点，表明该蛋白主要在植原体在植物寄主中生长时发挥作用。此外，在植物寄主中高表达的MSCL渗透通道的抑制剂部分抑制了植原体在植物体内的生长，表明渗透通道在植物寄主的生存中可能起着重要的作用。这些结果还表明，“寄主转换”机制的阐明可能有助于开发新的害虫防治措施。
Phytoplasmas are bacterial plant pathogens that have devastating effects on the yields of crops and plants worldwide. They are intracellular parasites of both plants and insects, and are spread among plants by insects. How phytoplasmas can adapt to two diverse environments is of considerable interest; however, the mechanisms enabling the “host switching” between plant and insect hosts are poorly understood. Here, we report that phytoplasmas dramatically alter their gene expression in response to “host switching” between plant and insect. We performed a detailed characterization of the dramatic change that occurs in the gene expression profile of Candidatus Phytoplasma asteris OY-M strain (approximately 33% of the genes change) upon host switching between plant and insect. The phytoplasma may use transporters, secreted proteins, and metabolic enzymes in a host-specific manner. As phytoplasmas reside within the host cell, the proteins secreted from phytoplasmas are thought to play crucial roles in the interplay between phytoplasmas and host cells. Our microarray analysis revealed that the expression of the gene encoding the secreted protein PAM486 was highly upregulated in the plant host, which is also observed by immunohistochemical analysis, suggesting that this protein functions mainly when the phytoplasma grows in the plant host. Additionally, phytoplasma growth in planta was partially suppressed by an inhibitor of the MscL osmotic channel that is highly expressed in the plant host, suggesting that the osmotic channel might play an important role in survival in the plant host. These results also suggest that the elucidation of “host switching” mechanism may contribute to the development of novel pest controls.
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影响因子：
4.4
作者：
Hren M;Nikolić P;Rotter A;Blejec A;Terrier N;Ravnikar M;Dermastia M;Gruden K
通讯作者：
Gruden K
影响因子：
30.8
作者：
Oshima, K;Kakizawa, S;Namba, S
通讯作者：
Namba, S
影响因子：
56.9
作者：
FRASER, CM;GOCAYNE, JD;VENTER, JC
通讯作者：
VENTER, JC
影响因子：
1.2
作者：
Kim, S;Watanabe, K;Watari, M
通讯作者：
Watari, M
影响因子：
3.5
作者：
Christensen, NM;Nicolaisen, M;Schulz, A
通讯作者：
Schulz, A