The IgA in milk induced by SARS-CoV-2 infection is comprised of mainly secretory antibody that is neutralizing and highly durable over time.
The IgA in milk induced by SARS-CoV-2 infection is comprised of mainly secretory antibody that is neutralizing and highly durable over time.
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DOI：
10.1371/journal.pone.0249723
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Powell RL
中科院分区：
文献类型：
--
作者：
Fox A;Marino J;Amanat F;Oguntuyo KY;Hahn-Holbrook J;Lee B;Zolla-Pazner S;Powell RL
大约10%感染SARS-CoV-2的婴儿将经历COVID-19疾病，需要高级护理。保护这一人群的一个潜在机制是通过先前感染者的乳汁进行被动免疫。我们和其他人已经报道了在母乳中存在SARS-CoV-2特异性抗体。我们现在报告了74名COVID-19康复参与者的牛奶中SARS-CoV-2伊加的患病率，发现89%的样本对Spike-specific伊加呈阳性。在这些样品的一个子集中，95%表现出稳健的伊加活性，如通过终点结合滴度测定的，其中50%被认为是高滴度。这些IgA阳性样品也对刺突特异性分泌抗体呈阳性。伊加抗体和分泌性抗体的水平被证明是强烈的正相关。分泌型伊加反应是占主导地位的牛奶样品中测试相比，IgG反应，这是目前在75%的样品，发现只有13%的情况下是高滴度。我们使用感染后4-6周和4-10个月获得的28对样本进行伊加持久性分析，发现所有样本均显示持续显著的尖峰特异性伊加，43%的供体显示伊加滴度随时间推移而增加。最后，检测了COVID-19和大流行前对照牛奶样本中是否存在中和抗体; 8个COVID-19样本中有6个显示出对刺突假型VSV的中和作用（IC 50范围，2.39- 89.4 ug/mL），而8个对照中只有1个。伊加结合和中和能力被认为是强烈的正相关。这些数据与公共卫生高度相关，不仅因为这些抗体对母乳喂养婴儿的保护能力，而且因为这些抗体作为COVID-19治疗药物的潜在用途，因为分泌型伊加在所有粘膜隔室中都含量很高。
Approximately 10% of infants infected with SARS-CoV-2 will experience COVID-19 illness requiring advanced care. A potential mechanism to protect this population is passive immunization via the milk of a previously infected person. We and others have reported on the presence of SARS-CoV-2-specific antibodies in human milk. We now report the prevalence of SARS-CoV-2 IgA in the milk of 74 COVID-19-recovered participants, and find that 89% of samples are positive for Spike-specific IgA. In a subset of these samples, 95% exhibited robust IgA activity as determined by endpoint binding titer, with 50% considered high-titer. These IgA-positive samples were also positive for Spike-specific secretory antibody. Levels of IgA antibodies and secretory antibodies were shown to be strongly positively correlated. The secretory IgA response was dominant among the milk samples tested compared to the IgG response, which was present in 75% of samples and found to be of high-titer in only 13% of cases. Our IgA durability analysis using 28 paired samples, obtained 4–6 weeks and 4–10 months after infection, found that all samples exhibited persistently significant Spike-specific IgA, with 43% of donors exhibiting increasing IgA titers over time. Finally, COVID-19 and pre-pandemic control milk samples were tested for the presence of neutralizing antibodies; 6 of 8 COVID-19 samples exhibited neutralization of Spike-pseudotyped VSV (IC50 range, 2.39–89.4ug/mL) compared to 1 of 8 controls. IgA binding and neutralization capacities were found to be strongly positively correlated. These data are highly relevant to public health, not only in terms of the protective capacity of these antibodies for breastfed infants, but also for the potential use of such antibodies as a COVID-19 therapeutic, given that secretory IgA is highly in all mucosal compartments.
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影响因子：
5.9
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通讯作者：
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影响因子：
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5.8
作者：
Fox A;Marino J;Amanat F;Krammer F;Hahn-Holbrook J;Zolla-Pazner S;Powell RL
通讯作者：
Powell RL
影响因子：
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作者：
Demers-Mathieu V;Underwood MA;Beverly RL;Nielsen SD;Dallas DC
通讯作者：
Dallas DC