Robust and Specific Secretory IgA Against SARS-CoV-2 Detected in Human Milk.
Robust and Specific Secretory IgA Against SARS-CoV-2 Detected in Human Milk.
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DOI：
10.1016/j.isci.2020.101735
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发表时间：
2020-11-20
期刊：
影响因子：
5.8
通讯作者：
Powell RL
中科院分区：
文献类型：
--
作者：
Fox A;Marino J;Amanat F;Krammer F;Hahn-Holbrook J;Zolla-Pazner S;Powell RL
尽管保护婴幼儿免受新冠肺炎侵袭对于限制社区传播、防止严重疾病和死亡至关重要，但尚未对母乳中的SARS-CoV-2免疫反应进行检测。在这里，来自8个新冠肺炎回收和7个新冠肺炎疑似捐赠者的牛奶样本被检测出与SARS-CoV-2刺突蛋白结合的抗体(Ab)。所有样本均表现出显著的特异性免疫球蛋白A反应，而80%的样本表现出显著的免疫球蛋白A和分泌型(S)抗体与受体结合域(RBD)结合。此外，67%的样本显示免疫球蛋白和/或免疫球蛋白M与RBD结合。IgA和SAB滴度高度相关，说明大多数IgA是SIgA。总体而言，这些数据表明，感染后母乳中SIgA主导的SARS-CoV-2抗体反应在绝大多数人中应该是预期的。进一步的研究是非常有必要的，以确定抗体的功能和开发提取的牛奶SIgA用于治疗的潜力。所有来自康复供者的乳汁都含有显著的SARS-CoV-2特异性免疫球蛋白A大多数免疫球蛋白A能结合受体结合区(重要的中和表位)大多数受体结合区特异性免疫球蛋白A是分泌型的(S)SIgA在粘膜中持久存在，因此有潜在的呼吸治疗儿科；免疫学；病毒学
The SARS-CoV-2 immune response in human milk has not yet been examined, although protecting infants and young children from COVID-19 is critical for limiting community transmission and preventing serious illness and death. Here, milk samples from eight COVID-19-recovered and seven COVID-19-suspected donors were tested for antibody (Ab) binding to the SARS-CoV-2 Spike protein. All samples exhibited significant specific IgA reactivity to the full Spike, whereas 80% exhibited significant IgA and secretory (s)Ab binding to the Receptor-Binding Domain (RBD). Additionally, 67% samples exhibited IgG and/or IgM binding to RBD. IgA and sAb titers were highly correlated, indicating most IgA to be sIgA. Overall, these data indicate that a robust sIgA-dominant SARS-CoV-2 Ab response in human milk after infection should be expected in a significant majority of individuals. Further research is highly warranted to determine Ab functionality and the potential for exploiting extracted milk sIgA for therapeutic use. All milk from recovered donors contained significant SARS-CoV-2-specific IgA Most IgA could bind the Receptor-Binding Domain (important neutralization epitope) Most Receptor-Binding Domain-specific IgA was in secretory (s) form sIgA is durable in the mucosa, and thus potentially as a respiratory therapeutic Pediatrics; Immunology; Virology
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