The Reparative Function of MMP13 in Tertiary Reactionary Dentinogenesis after Tooth Injury.
The Reparative Function of MMP13 in Tertiary Reactionary Dentinogenesis after Tooth Injury.
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DOI：
10.3390/ijms25020875
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发表时间：
2024-01-10
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
MMP 13基因表达在矿化牙髓细胞（DPC）中增加高达2000倍，先前的研究表明，整体MMP 13缺失导致牙本质表型的关键改变，影响牙本质小管规则性，成牙本质细胞栅栏，并显着减少牙本质体积。一系列年龄的全球MMP 13-KO和野生型小鼠的臼齿受损，以刺激反应性三级牙本质形成。使用组织学、免疫组织化学、micro-CT和qRT-PCR定性和定量测量反应，以评估沉积的牙本质的性质和体积的变化以及机械联系。MMP 13损失影响反应性三级牙本质的质量和体积后，牙尖损伤和减少巢蛋白的表达在非暴露损伤模型，以及MMP 13和Wnt反应基因Axin 2之间的机械联系。急性牙髓损伤和牙髓暴露于小鼠牙齿中的口腔液体显示MMP 13在体内上调，Mmp 8，Mmp 9和Mmp 13的基因表达明显增加。这些结果表明，MMP 13参与了体内牙齿损伤后三级反应性牙本质的形成，可能在牙本质-牙髓修复过程中充当牙髓中的关键分子。
MMP13 gene expression increases up to 2000-fold in mineralizing dental pulp cells (DPCs), with research previously demonstrating that global MMP13 deletion resulted in critical alterations in the dentine phenotype, affecting dentine–tubule regularity, the odontoblast palisade, and significantly reducing the dentine volume. Global MMP13-KO and wild-type mice of a range of ages had their molar teeth injured to stimulate reactionary tertiary dentinogenesis. The response was measured qualitatively and quantitatively using histology, immunohistochemistry, micro-CT, and qRT-PCR in order to assess changes in the nature and volume of dentine deposited as well as mechanistic links. MMP13 loss affected the reactionary tertiary dentine quality and volume after cuspal injury and reduced Nestin expression in a non-exposure injury model, as well as mechanistic links between MMP13 and the Wnt-responsive gene Axin2. Acute pulpal injury and pulp exposure to oral fluids in mice teeth showed upregulation of the MMP13 in vivo, with an increase in the gene expression of Mmp8, Mmp9, and Mmp13 evident. These results indicate that MMP13 is involved in tertiary reactionary dentine formation after tooth injury in vivo, potentially acting as a key molecule in the dental pulp during dentine–pulp repair processes.
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影响因子：
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作者：
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通讯作者：
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