Development of an antimicrobial peptide-loaded mineralized collagen bone scaffold for infective bone defect repair.
Development of an antimicrobial peptide-loaded mineralized collagen bone scaffold for infective bone defect repair.
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开发用于修复感染性骨缺损的负载抗菌肽的矿化胶原骨支架
DOI：
10.1093/rb/rbaa015
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发表时间：
2020-10
影响因子：
6.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
He Y;Jin Y;Ying X;Wu Q;Yao S;Li Y;Liu H;Ma G;Wang X
感染性骨缺损的修复是临床工作中的一大挑战。开发一种具有良好成骨性能和长期抗菌活性的骨支架材料对局部抗感染和骨再生具有重要意义。以聚乳酸-羟基乙酸共聚物（PLGA）微球为载体，分别负载抗菌肽Pac-525和KSL-W，制备了一种矿化胶原多孔支架材料。结果表明，包埋有光滑致密PLGA微球的MC支架对细胞生长有积极的影响，并具有抗菌性能。通过毒性分析、细胞形态和增殖分析以及碱性磷酸酶活性评价，抗菌支架具有良好的生物相容性和成骨活性。对金黄色葡萄球菌和大肠杆菌的抗菌性能评价表明，从支架中缓释Pac-525或KSL-W可以长期抑制上述细菌的生长。结果表明，载抗菌肽的MC骨支架具有良好的抗菌和成骨活性，为感染性骨缺损的治疗提供了良好的前景。
Abstract The repair of infective bone defects is a great challenge in clinical work. It is of vital importance to develop a kind of bone scaffold with good osteogenic properties and long-term antibacterial activity for local anti-infection and bone regeneration. A porous mineralized collagen (MC) scaffold containing poly(d,l-lactide-co-glycolic acid) (PLGA) microspheres loaded with two antibacterial synthetic peptides, Pac-525 or KSL-W was developed and characterized via scanning electron microscopy (SEM), porosity measurement, swelling and mechanical tests. The results showed that the MC scaffold embedded with smooth and compact PLGA microspheres had a positive effect on cell growth and also had antibacterial properties. Through toxicity analysis, cell morphology and proliferation analysis and alkaline phosphatase evaluation, the antibacterial scaffolds showed excellent biocompatibility and osteogenic activity. The antibacterial property evaluated with Staphylococcus aureus and Escherichia coli suggested that the sustained release of Pac-525 or KSL-W from the scaffolds could inhibit the bacterial growth aforementioned in the long term. Our results suggest that the antimicrobial peptides-loaded MC bone scaffold has good antibacterial and osteogenic activities, thus providing a great promise for the treatment of infective bone defects.
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影响因子：
9.7
作者：
Offeddu, G. S.;Ashworth, J. C.;Oyen, M. L.
通讯作者：
Oyen, M. L.
长效抗菌肽负载PLGA/壳聚糖复合微球的制备
DOI：
10.3390/molecules22101637
发表时间：
2017-09-29
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Li Y;Na R;Wang X;Liu H;Zhao L;Sun X;Ma G;Cui F
通讯作者：
Cui F
影响因子：
4.6
作者：
Di Luca A;Ostrowska B;Lorenzo-Moldero I;Lepedda A;Swieszkowski W;Van Blitterswijk C;Moroni L
通讯作者：
Moroni L
影响因子：
5.6
作者：
Glenske K;Donkiewicz P;Köwitsch A;Milosevic-Oljaca N;Rider P;Rofall S;Franke J;Jung O;Smeets R;Schnettler R;Wenisch S;Barbeck M
通讯作者：
Barbeck M
影响因子：
6.7
作者：
Nelson C;Khan Y;Laurencin CT
通讯作者：
Laurencin CT