Interaction of Ferromagnetic Shape Memory Alloys and RGD Peptides for Mechanical Coupling to Cells: from Ab Initio Calculations to Cell Studies
Interaction of Ferromagnetic Shape Memory Alloys and RGD Peptides for Mechanical Coupling to Cells: from Ab Initio Calculations to Cell Studies
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铁磁形状记忆合金和 RGD 肽的相互作用，用于细胞机械耦合：从从头计算到细胞研究
DOI：
10.1002/adfm.201201789
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发表时间：
2013
影响因子：
19
通讯作者：
S.G. Mayr
中科院分区：
文献类型：
--
作者：
M. Zink;F. Szillat;U. Allenstein;S.G. Mayr
由于它们的磁-机械耦合和生物相容性，Fe-Pd基铁磁形状记忆合金是一种非常有前途的材料，可用作生物医学环境中的非接触式磁-机械传感器。对于在细胞和组织致动器或应变传感器中的使用，足够的粘附以介导应变显然构成先决条件。由于RGD序列是哺乳动物细胞最重要的结合基序，它们表达以促进粘附，因此探索了RGD涂层实现这一目标的潜力。采用大规模密度泛函理论计算，澄清了RGD和Fe-Pd表面之间的键合物理学，其特征在于O和N原子与Fe的配位键，伴随着静电贡献。实验证实的关于粘附的理论预测表明，RGD是Fe-Pd表面的合适应变介质。在细胞方面，RGD涂层的Fe-Pd的良好粘附特性表现在细胞形态和铺展行为中。证明了RGD和Fe-Pd之间的粘附力超过了细胞对RGD涂层施加的粘附力，以及作用于整合素键的牵引力，这些发现为组织工程和再生医学领域中作为细胞和组织致动器或传感器的新型应用铺平了道路。
Due to their magneto‐mechanical coupling and biocompatibility, Fe‐Pd based ferromagnetic shape memory alloys are a highly promising materials class for application as contact‐less magneto‐mechanical transducers in biomedical environments. For use in cell and tissue actuators or strain sensors, sufficient adhesion to mediate strains clearly constitutes a prerequisite. As the RGD sequence is the most important binding motif for mammalian cells, which they express to facilitate adhesion, the potential of RGD coatings to achieve this goal is explored. Employing large‐scale density functional theory calculations the physics of bonding between RGD and Fe‐Pd surfaces, which is characterized by coordinate bonds of O and N atoms to Fe, accompanied by electrostatic contributions, is clarified. Theoretical predictions on adhesion, that are confirmed experimentally, suggest RGD as suitable strain mediator to Fe‐Pd surfaces. On the cell side, favorable adhesion properties of RGD‐coated Fe‐Pd are manifested in cell morphology and spreading behavior. Demonstrating that the adhesion forces between RGD and Fe‐Pd exceed those exerted by cells to the RGD coating, as well as traction forces acting onto integrin bonds, the findings pave the way for novel type of applications as cell and tissue actuator or sensor within the areas of tissue engineering and regenerative medicine.
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