Immunological pathways of macrophage response to Brucella ovis infection.
Immunological pathways of macrophage response to Brucella ovis infection.
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巨噬细胞对绵羊布鲁氏菌感染反应的免疫学途径
DOI：
10.1177/1753425920958179
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发表时间：
2020-10
期刊：
影响因子：
3.2
通讯作者：
Jiao H
中科院分区：
文献类型：
--
作者：
Zhou Z;Gu G;Luo Y;Li W;Li B;Zhao Y;Liu J;Shuai X;Wu L;Chen J;Fan C;Huang Q;Han B;Wen J;Jiao H
由于绵羊布鲁氏菌致病性的分子机制尚不完全清楚，我们应用转录组方法鉴定了绵羊布鲁氏菌感染的RAW264.7巨噬细胞中的差异表达基因（DEG）。通过京都基因与基因组百科全书（KEGG）和基因本体论（GO）功能富集分析鉴定出与免疫通路相关的DEG。进行定量实时 PCR (qRT-PCR) 以验证转录组测序数据。与模拟组相比，我们总共鉴定出绵羊双歧杆菌感染组中有 337 个上调 DEG 和 264 个下调 DEG。通过KEGG分析富集前20条通路，并通过功能富集分析20条GO，对涉及分子功能、细胞成分、生物过程等的DEG进行功能富集分析，揭示了RAW264.7巨噬细胞响应绵羊B. ovis感染的多种免疫通路，包括炎症反应、免疫系统过程、免疫反应、细胞因子活性、趋化性、趋化因子介导的信号通路、趋化因子活性和CCR趋化因子受体绑定。 qRT-PCR结果显示Ccl2 (ENSMUST00000000193)、Ccl2 (ENSMUST00000124479)、Ccl3 (ENSMUST00000001008)、Hmox1 (ENSMUST00000005548)、Hmox1 (ENSMUST00000159631)、Cxcl2 (ENSMUST00000075433)、Cxcl2 (ENSMUST00000200681)、Cxcl2 (ENSMUST00000200919) 和 Cxcl2 (ENSMUST00000202317)。我们的研究结果首先阐明了绵羊布鲁氏菌诱导宿主免疫反应的途径，这可能为揭示细菌与宿主的相互作用和阐明绵羊布鲁氏菌的致病机制奠定基础。
As the molecular mechanisms of Brucella ovis pathogenicity are not completely clear, we have applied a transcriptome approach to identify the differentially expressed genes (DEGs) in RAW264.7 macrophage infected with B. ovis. The DEGs related to immune pathway were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) functional enrichment analysis. Quantitative real-time PCR (qRT-PCR) was performed to validate the transcriptome sequencing data. In total, we identified 337 up-regulated and 264 down-regulated DEGs in B. ovis-infected group versus mock group. Top 20 pathways were enriched by KEGG analysis and 20 GO by functional enrichment analysis in DEGs involved in the molecular function, cellular component, and biological process and so on, which revealed multiple immunological pathways in RAW264.7 macrophage cells in response to B. ovis infection, including inflammatory response, immune system process, immune response, cytokine activity, chemotaxis, chemokine-mediated signaling pathway, chemokine activity, and CCR chemokine receptor binding. qRT-PCR results showed Ccl2 (ENSMUST00000000193), Ccl2 (ENSMUST00000124479), Ccl3 (ENSMUST00000001008), Hmox1 (ENSMUST00000005548), Hmox1 (ENSMUST00000159631), Cxcl2 (ENSMUST00000075433), Cxcl2 (ENSMUST00000200681), Cxcl2 (ENSMUST00000200919), and Cxcl2 (ENSMUST00000202317). Our findings firstly elucidate the pathways involved in B. ovis-induced host immune response, which may lay the foundation for revealing the bacteria–host interaction and demonstrating the pathogenic mechanism of B. ovis.
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影响因子：
3
作者：
Du, Zhi-Qiang;Li, Xin;Wang, Jian-Ying
通讯作者：
Wang, Jian-Ying
影响因子：
1.8
作者：
Graciela Diaz, Alejandra;Alejandra Quinteros, Daniela;Marcela Estein, Silvia
通讯作者：
Marcela Estein, Silvia
影响因子：
2.8
作者：
Kumar, Sanjay;Tuteja, Urmil;Kumar, Om
通讯作者：
Kumar, Om
影响因子：
1.2
作者：
Fiorentino MA;Paolicchi FA;Campero CM;Barbeito CG
通讯作者：
Barbeito CG
影响因子：
2.7
作者：
Didion JP;Martin M;Collins FS
通讯作者：
Collins FS