Th17 differentiation is the default program for DPP2-deficient T-cell differentiation.
Th17 differentiation is the default program for DPP2-deficient T-cell differentiation.
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DOI：
10.1002/eji.201041157
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发表时间：
2011-06
影响因子：
5.4
通讯作者：
Huber, Brigitte T.
中科院分区：
文献类型：
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作者：
Mele, Deanna A.;Sampson, James F.;Huber, Brigitte T.
DPP 2（二肽基肽酶2）是一种N-末端二肽酶，维持淋巴细胞处于静息状态所需。产生具有DPP 2的T细胞特异性敲低（kd）的突变小鼠（lck-DPP 2kd）并分析其表型。与同窝对照组相比，在这些小鼠中观察到正常胸腺细胞发育和外周T细胞比例适度增加。有趣的是，外周T细胞在体外TCR刺激后过度活跃，尽管它们不表达任何活化标志物。此外，lck-DPP 2kd小鼠的初始CD 4+和CD 8 + T细胞中的CD 3交联主要导致IL-17产生。类似地，突变T细胞在体内引发和体外抗原特异性再刺激后主要分泌IL-17。这些数据表明，IL-17产生是在不存在DPP 2的情况下T细胞分化的默认程序。因此，DPP 2似乎对静止的T细胞施加了一个阈值，阻止它们漂移进入细胞周期。
DPP2 (dipeptidyl peptidase 2) is an N-terminal dipeptidase, required for maintaining lymphocytes in a resting state. Mutant mice with T cell-specific knock-down (kd) of DPP2 (lck-DPP2 kd) were generated and analyzed for their phenotype. Normal thymocyte development and a modest increase in the proportions of peripheral T cells were observed in these mice compared to littermate controls. Interestingly, the peripheral T cells were hyperactive upon TCR stimulation in vitro, although they did not express any activation markers. Furthermore, CD3-crosslinking in the naive CD4+ and CD8+ T cells of lck-DPP2 kd mice resulted mainly in IL-17 production. Similarly, the mutant T cells secreted primarily IL-17 after in vivo priming and in vitro antigen-specific restimulation. These data suggest that IL-17 production is the default program for T cell differentiation in the absence of DPP2. Thus, DPP2 seems to impose a threshold for quiescent T cells, preventing them from drifting into cell cycle.
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影响因子：
64.5
作者：
Ivanov, Ivaylo I.;McKenzie, Brent S.;Littman, Dan R.
通讯作者：
Littman, Dan R.
DOI：
10.1084/jem.186.8.1223
发表时间：
1997-10-20
期刊：
The Journal of experimental medicine
影响因子：
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作者：
Brocker T
通讯作者：
Brocker T
影响因子：
30.5
作者：
Doreau, Agnes;Belot, Alexandre;Bonnefoy-Berard, Nathalie
通讯作者：
Bonnefoy-Berard, Nathalie
影响因子：
100.3
作者：
Dong, C
通讯作者：
Dong, C
影响因子：
30.5
作者：
通讯作者：
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