Identification and molecular characterization of Serratia marcescens phages vB_SmaA_2050H1 and vB_SmaM_2050HW
Identification and molecular characterization of Serratia marcescens phages vB_SmaA_2050H1 and vB_SmaM_2050HW
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粘质沙雷氏菌噬菌体 vB_SmaA_2050H1 和 vB_SmaM_2050HW 的鉴定和分子表征
DOI：
10.1007/s00705-019-04169-1
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发表时间：
2019-02
影响因子：
2.7
通讯作者：
Zhao Xiangna
中科院分区：
文献类型：
--
作者：
Tian Changyu;Zhao Jiangtao;Zhang Zheng;Chen Xiao;Wei Xiao;Li Huan;Lin Weishi;Ke Yuehua;Hu Lingfei;Jiang Aimin;Feng Ruo;Yang Wenhui;Jing Ying;Yuan Jing;Luo Yanping;Zhao Xiangna
粘质沙雷氏菌是一种杆状革兰氏阴性细菌，可引起医院获得性感染。噬菌体是其致病细菌宿主的天然对手，可以替代传统的抗生素治疗。在本研究中，两个S.从中国的两种不同的废物样品中分离出marcescens特异性噬菌体vB_SmaA_2050H1和vB_SmaM_2050HW。噬菌体空斑试验，透射电子显微镜，主机范围的确定，和一步生长曲线分析进行了两个ESTA。vB_SmaA_2050H1被分类为属于阿克曼病毒科，vB_SmaM_2050HW被分类为属于肌病毒科。一步生长曲线分析表明，vB_SmaA_2050H1的潜伏期和上升期分别为80 min和50 min，爆发大小约为每个感染细胞103个噬菌体颗粒。对于vB_SmaM_2050HW，确定潜伏期和上升期分别为40 min和60 min，爆发大小为每个感染细胞约110个噬菌体颗粒。vB_SmaA_2050H1感染10株（66.67%）。粘质杆菌菌株，而vB_SmaM_2050HW感染12个菌株（80%）。每个噬菌体基因组的全基因组测序和注释显示，vB_SmaA_2050 H1和vB_SmaM_2050 HW的基因组大小分别为159，631 bp和276，025 bp，各自的基因组含有213和363个推定的开放阅读框。基因组序列分析表明，vB_SmaA_2050H1是ViI样家族的成员，而vB_SmaM_2050HW是一种新的毒性噬菌体。这些发现为进一步了解S.粘质噬菌体
Serratia marcescens is a rod-shaped, Gram-negative bacterium causing nosocomially acquired infections. Bacteriophages are natural opponents of their pathogenic bacterial hosts and could be an alternative to traditional antibiotic treatments. In this study, two S. marcescens-specific bacteriophages, vB_SmaA_2050H1 and vB_SmaM_2050HW, were isolated from two different waste samples in China. Phage plaque assays, transmission electron microscopy, host-range determination, and one-step growth curve analyses were performed for both phages. vB_SmaA_2050H1 was classified as belonging to the family Ackermannviridae, and vB_SmaM_2050HW was classified as belonging to the family Myoviridae. One-step growth curve analysis showed that the latent and rise period of vB_SmaA_2050H1 were 80 min and 50 min, respectively, with a burst size of approximately 103 phage particles per infected cell. For vB_SmaM_2050HW, latent and rise periods of 40 min and 60 min, respectively, were determined, with a burst size of approximately 110 phage particles per infected cell. vB_SmaA_2050H1 infected 10 of the 15 (66.67%) S. marcescens strains tested, while vB_SmaM_2050HW infected 12 (80%) of the strains. Whole-genome sequencing and annotation of each of the phage genomes revealed genome sizes of 159,631 bp and 276,025 bp for vB_SmaA_2050H1 and vB_SmaM_2050HW, respectively, with the respective genomes containing 213 and 363 putative open reading frames. Sequence analysis of the genomes revealed that vB_SmaA_2050H1 is a member of the ViI-like family, while vB_SmaM_2050HW is a novel virulent bacteriophage. These findings provide further insights into the genomic structures of S. marcescens bacteriophages.
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影响因子：
4.8
作者：
Denyes JM;Krell PJ;Manderville RA;Ackermann HW;She YM;Kropinski AM
通讯作者：
Kropinski AM
DOI：
10.3390/v9040070
发表时间：
2017-04-03
期刊：
Viruses
影响因子：
--
作者：
Adriaenssens E;Brister JR
通讯作者：
Brister JR
影响因子：
4.8
作者：
Hooton SP;Timms AR;Rowsell J;Wilson R;Connerton IF
通讯作者：
Connerton IF
影响因子：
2.1
作者：
Meczker, Katalin;Doemoetoer, Dora;Kovacs, Tamas
通讯作者：
Kovacs, Tamas
影响因子：
4.2
作者：
Peng F;Mi Z;Huang Y;Yuan X;Niu W;Wang Y;Hua Y;Fan H;Bai C;Tong Y
通讯作者：
Tong Y