Effect of Nourishing "Yin"-Removing "Fire" Chinese Herbal Mixture on Hypothalamic NKB/NK3R Expression in Female Precocious Rats.
Effect of Nourishing "Yin"-Removing "Fire" Chinese Herbal Mixture on Hypothalamic NKB/NK3R Expression in Female Precocious Rats.
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DOI：
10.1155/2014/217424
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Tian Z
中科院分区：
文献类型：
--
作者：
Wang S;Zhu L;Yu J;Tian Z
的目标。本研究旨在探讨养阴除火中药合剂对雌性性早熟模型大鼠下丘脑NKB/NK3R表达的影响。材料与方法。雌性Sprague-Dawley大鼠随机分为正常(N)组、中枢性性早熟（CPP）模型(M)组、中草药合剂（CHM）组和生理盐水（MS）组。大鼠出生后第5天皮下注射300 μg建立CPP模型。CHM组和MS组大鼠自出生后第15天起连续给予养阴除火中药合剂或生理盐水。采用real-time PCR、western blot、免疫荧光组织化学检测下丘脑NKB/NK3R的表达。结果。与M组和MS组相比，CHM组阴道开放天数和两次正常发情周期的建立均延迟。CHM组下丘脑弓状核（ARC）和内侧视前区（MPO） NKB/NK3R mRNA和蛋白的表达在发育期当天较M和MS组显著降低。结论。这些结果表明，NKB/NK3R信号通路可能参与了合剂治疗对CPP的影响。
Aim. The present study aims to investigate the effects of nourishing “Yin”-removing “Fire” Chinese herb mixture on the hypothalamic NKB/NK3R expression in female precocious model rats. Materials and Methods. Female Sprague-Dawley rats were randomly divided into four groups: normal (N), central precocious puberty (CPP) model (M), CPP fed with Chinese herbal mixture (CHM), and CPP fed with normal saline (MS). Rats on postnatal day 5 were given a single subcutaneous injection of 300 μg to establish CPP model rats. Rats of CHM and MS groups were continuously administered with nourishing “Yin”-removing “Fire” Chinese herb mixture or saline since postnatal day 15. The expressions of hypothalamic NKB/NK3R were detected by means of real-time PCR, western blot, and immunofluorescence histochemistry. Results. The day of vaginal opening and establishment of two regular estrous cycles were delayed in the CHM group compared with M and MS groups. The expression of hypothalamic NKB/NK3R mRNA and protein in the arcuate nucleus (ARC) and medial preoptic (MPO) area were decreased significantly in the CHM group compared with the M and MS groups on the day of onset-puberty. Conclusions. These results indicate that the NKB/NK3R signaling pathway might be involved in the effect of herbal mixture treatment on CPP.
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影响因子：
3.3
作者：
Krajewski, S. J.;Burke, M. C.;Anderson, M. J.;McMullen, N. T.;Rance, N. E.
通讯作者：
Rance, N. E.
影响因子：
1.8
作者：
Noritake, Ken-ichi;Matsuoka, Toshiki;Tsukamura, Hiroko
通讯作者：
Tsukamura, Hiroko
影响因子：
5.8
作者：
Lazar, L;Kauli, R;Phillip, M
通讯作者：
Phillip, M
影响因子：
4.8
作者：
Dellovade, TL;Merchenthaler, I
通讯作者：
Merchenthaler, I
影响因子：
2.5
作者：
MORISHITA, H;TAKEMOTO, M;AONO, T
通讯作者：
AONO, T